The invention provides a preparation method of human adipose
mesenchymal stem cell exosome for treating
myocardial infarction, and relates to the technical field of
biology. The preparation method ofthe human
mesenchymal stem cell exosome for treating
myocardial infarction provided by the invention comprises the following steps: culturing human adipose mesenchymal stem cells, and collecting a
cell culture solution; and carrying out
differential centrifugation on the
cell culture solution, collecting the supernatant, carrying out ultracentrifugation on the supernatant to obtain target microspheres, and then filtering, purifying and sterilizing the target microspheres to obtain the human adipose
mesenchymal stem cell exosome for treating
myocardial infarction. According to the present invention, the
advantage is that the human adipose mesenchymal
stem cell exosome is prepared by culturing human adipose mesenchymal stem cells, and the human adipose mesenchymal
stem cell exosome containsvarious RNAs and cytokines, so that infarcted myocardial cells are effectively repaired, the cardiac function is enhanced, compensatory myocardial load is reduced, and
heart failure is relieved; and atreatment
medicine or a
treatment strategy is provided for myocardial
infarction.