45results about "Laboratory glasswares" patented technology

The present invention pertains to a device and method to measure thermo-optical, preferably thermophoretic, characteristics of particles in a solution. The method comprises the steps of: (a) providing a sample probe comprising marked particles in a solution; (b) providing a temperature control system for creating a temperature gradient within said sample probe by contact heating, electrical heating and/or cooling; (c) detecting the marked particles at a first time; (d) creating a temperature gradient within the sample probe by means of the temperature control system; (e) detecting the marked particles in the sample probe at a, preferably predetermined, second time and/or at a predetermined location within the temperature gradient, and (f) characterizing the particles based on said two detections.

A device for providing pipette tips includes a plate-shaped carrier with a base surface, holes in the carrier perpendicular to the base surface, at least one vertically running cut-out in the outer sides of two opposing narrow sides of the carrier to accommodate two vertical guide ribs at a distance from each other, pipette tips in the holes, a bottom part with sidewalls that extend upwards from a standing surface, said sidewalls surrounding a rectangular seat for the carrier with the inserted pipette tips, wherein two opposing first sidewalls project further upward from the standing surface than to opposing second sidewalls, a supporting mechanism on the inner sides of at least two opposing sidewalls to support the carrier in a horizontal alignment at the bottom edges of narrow sides, and two vertical guide ribs engaging in the at least one cut-out when the carrier is placed on the supporting mechanism.

There are provided a chemical treatment cartridge capable of executing various extraction processes with ease and a method of using the same. There are formed recesses, each in a predetermined shape, in the back surface of an elastic member. The recesses create empty spaces between the cartridge substrate and the elastic member, thereby making up a well for receiving a sample, a well for pre-removing unnecessary moisture, a well for accommodating the sample at the time of distillation, a well for storing impurities therein, a well for taking out an object constituent, a flow path interconnecting the side surface of the cartridge and the well, a flow path interconnecting the well and the well, a flow path interconnecting the well and the well, a flow path interconnecting the well and the well and a flow path interconnecting the well and the well.

The invention relates to mutant forms of Msp. The invention also relates to polynucleotide characterisation using Msp.

Devices, methods and systems for low volume microarray processing are disclosed. The microarray devices preferably include a plurality of reactant sites on a reactant surface. The reactant sites include reactants that operate to capture one or more selected analytes that can then be detected based on an electromagnetic signal, e.g., fluorescence, that is emitted by each analyte in response to excitation energy incident on the microarray device. Mixing and/or distribution of the analyte sample over the reactant surface is accomplished by tilting the reactant surface such that the analyte sample flows over the reactant surface under the force of gravity. The tilting is performed such that a portion of the analyte sample accumulates in a bead along a first edge of the reactant surface. The reactant surface is then tilted in a different direction such that a portion of the analyte sample flows over the reactant surface and accumulates at a second edge. The reactant surface preferably generates sufficient capillary forces with the analyte sample such that the analyte sample is retained on the reactant surface. The tilting and resultant flow may be performed as many times as necessary to obtain the desired mixing and/or distribution of analyte sample over the reactant surface.

The invention provides a micro-fluidic chip for preparing microspheres and a using method of the micro-fluidic chip. The micro-fluidic chip is mainly compounded by a chip body, a cooling chip and a volatilizing and exhausting chip. The micro-fluidic chip is characterized by comprising two V-shaped mixing pipelines I and II which are integrally U-shaped, circular cooling double U-shaped pipelines, a sizing and collecting pond which is arranged on one side of the circular cooling double U-shaped pipelines, an outlet and the like. The invention further provides a using and operating method of the chip. The micro-fluidic chip provided by the invention has the beneficial effects that the prepared microspheres are small in particle size dispersity and high in dimensional precision, compound microspheres with more than two components can be quickly prepared, the production efficiency is high and the operation is convenient and simple.

Provided are a microfluidic chip for multiple bioassay and a method of manufacturing the same. The method includes: forming microfluidic channels by coupling a channel structure having grooves for sample channels and probe channels in a bottom surface and a substrate; forming probe immobilization regions at intersections between the probe channels and the sample channels; and forming blocking walls in the probe channels before and after each of the probe immobilization regions to prevent mixing of target sample. Since the probes are immobilized in the microfluidic channels after the formation of the microfluidic channels, difficulty in connecting fluidic channels after the immobilization of probes on a substrate can be removed. In addition, a microfluidic platform for a multiple bioassay into which a plurality of samples can be simultaneously loaded can be formed.

The invention discloses a reaction incubation device, an immunity analyzer and a reaction incubation method. The reaction incubation device comprises a reaction unit (10) and a transfer unit (20), wherein the reaction unit (10) is use for bearing and incubating a reaction container; the transfer unit (20) is used for removing the reaction container in and out of the reaction unit (10); the reaction unit (10) comprises a rotating device (11); the rotating device (11) is provided with an incubation position; the incubation position moves a predetermined angle theta at an interval of fixed time T along with the rotating device (11); and the transfer unit (20) removes the reaction container out of the incubation position according to variable incubation time t1. Flexible and variable incubation time can be simply and efficiently achieved, and the problems that control is complicated, the reliability is low and high-speed automation is not easy to achieve in the prior art to achieve the variable incubation time are effectively solved.

Described herein are embossing methods for forming pico liter well arrays (and other structures), arrangements of such pico liter well arrays, for example, in arrays of pico liter well areas, and methods of forming templates for such embossing. Also described as wells with a refractive index similar to that of an aqueous medium used for supporting cells in the wells.

The invention discloses an integration micro-fluidic chip for immune analysis research. The chip is mainly composed of a concentration gradient generation unit, an equal allocation unit and a micropore reaction chamber matrix with the diameter of 5.8mm and the depth of 100 mu, and has small volume and large specific surface area; the arrangement of the micropore reaction chamber matrix is consistent with the arrangement of a traditional porous plate, and the micropore matrix can be applied to a standard microplate reader for detecting and gathering analysis data. According to the invention, the operations of absorption and fixation of antibodies, antigens and enzyme during immune analysis operation, the preparation of sample solutions with different concentrations, liquid replacement, washing and the other operations are all integrated on a chip and are completed automatically according to the process creatively; the reagent consumption can be lowered obviously; the analysis time can be shortened; and the efficiency can be improved. Compared with the traditional porous plate technology, according to the invention, the complex manual operations of sample solution preparation, washing and the like are omitted, and the integration micro-fluidic chip for immune analysis research is compatible with the standard microplate reader on the detecting method.

The present invention relates to a heat sealable member comprising a sheet of heat sealable material to a multiwell plate, wherein the sheet is mounted in a frame, and wherein said frame is sized to fit over a multiwell plate so that the sheet is contacted with at least one of the rims of the top of the plate and the individual wells; as well as to the use of the heat sealable member with automatic plate handling apparatus, especially in PCR processes. The present invention further relates to a method for sealing multiwell plates.

The present invention relates to a microfluidic device used for cell motility screening and chemotaxis testing which comprises microfluidic channels and chambers. Cells which can secret a chemoattractant or chemorepellent are selectively planted in the microfluidic device and a stable chemoattractant or chemorepellent gradient can be established in the channels.

The invention discloses a high-efficiency single-cell capture and rapid single-cell secretory protein detection platform and method. The detection platform is composed of three parts of a single-cellcapture and liquid drop incubation micro-fluidic control chip, a secretory protein capture glass plate and a chip clamp; and the single-cell capture and liquid drop incubation micro-fluidic control chip is provided with two layers of structures, namely a single-cell capture and liquid drop incubation layer and an isolation valve layer. The platform can perform single-cell capture with high efficiency and fastness, micro-droplets containing single cells are rapidly and stably generated through the addition of the isolation phase, and long-term liquid drop incubation can be carried out; and meanwhile, the high activity of the single cells is ensured, the chip is combined with a detection antibody array glass plate of secretory protein, and the high-flux, rapid and high-sensitivity detectionof the single-cell secretory protein can be realized.

The invention relates to a full automatic extraction device, which is used for extraction experiment in a chemical laboratory. The device comprises a shell, a getter device, an extraction container and a tap funnel which are fixedly arranged on the shell, wherein the tap funnel is positioned on the upper part of the extraction container; the extraction container is communicated with the getter device through an air duct and connected positions are all closed; the bottom end of the extraction container is provided with an imbibition long tube; the imbibition long tube passes through an opening on the top part of the tap funnel to the bottom of the tap funnel; the bottom of the tap funnel is provided with a drain pipe; the drain pipe is provided with a control valve; and the getter device is arranged in the shell, an outlet of the getter device is connected with a vent pipe, and the vent pipe extends outside the shell. The device adopts batch type vacuumizing principle, guides organic phase and aqueous phase to come and go rapidly in the extraction container and the tap funnel so as to make a target compound sufficiently transfer from the aqueous phase to the organic phase, thereby realizing liquid-liquid extraction and separation. The device can replace manual operation and save time and labor, and has good uniformity, an experimenter cannot breathe harmful gas, so the device is favorable for protecting human body from harm.

Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass frit.

Methods and devices for microfluidic detection of a biological maker in a biospecimen collected from a subject are disclosed. The microfluidic devices include nanoparticle-based nanosensors comprising supramolecular recognition sequences, protease consensus sequences, post-translationally modifiable sequences, or sterically hindered benzylether bonds for specific interaction with a biological marker. Also disclosed are particular nanosensors for detecting cytokines, and other proteins based upon supramolecular recognition without chemical modification or enzymatic cleavage.

The present disclosure relates to a biosensor capable of measuring the total concentration of one or a plurality of amino acids with the use of a reaction surface comprising one or a plurality of metabolic enzymes or functional fragments thereof, but wherein the reaction surface does not comprise an electrode or electrically conductive support. In some embodiments, the biosensor comprises use of a thermophilic bacterial metabolic enzyme immobilized or attached to the reaction surface.

A simple and accurate separation purification method for object biomolecules or bio-associated substance through a particulate aggregative reaction is provided. A microfluidic circuit utilizing a particulate aggregate as a detection marker in a nonfluorescence method such as an electrochemical method or surface plasmon resonance is provided. The separation purification method comprises the steps of forming a particulate aggregate by a reaction between particulates modified with a labeling substance specifically reacting with a label object substance and biomolecules or a bio-associated substance containing the label object substance and separation-purifying the particulate aggregate. The microfluidic circuit utilizes this separation purification method.

The invention discloses a preparation method and applications of a three-dimensional structure-based nanoarray biochip. The method comprises the following steps: (1) treating a gold film through silane solution containing sulfhydryl group; (2) treating the gold film, treated by the step (1), through acid solution; (3) attaching an anodized aluminum thin film of a through hole onto the gold film treated in the step (2); (4)dipping the gold film, obtained in the step (3), into SiCl4, n-hexane, hexane-methanol mixed solution, ethanol and water sequentially (circularly carrying out the processes); (5) treating a gold substrate, obtained in the step (4), through acid solution. According to the method, a nanotube (column) array with the three-dimensional structure is easily prepared on the substrate, the size of the tube (column) is controllable, the three-dimensional structure-based nanoarray biochip can be used as a biochip, and the high-sensitivity and label-free biodetection can be carried out.

The invention provides a microfluidic chip and a microfluidic drive and control system thereof, and belongs to the technical field of micromachine. The microfluidic chip includes a control layer, a PDMS film layer, a fluidic channel layer and a glass substrate. The fludic channel layer is provided with one main channel and three sorting channels; a switch of the sorting channels are controlled bya thermal expansion micovalve of the control layer; the microfluidic drive and control system includes an observing device and a fluidic control device. According to the microfluidic chip and the microfluidic drive and control system thereof provided by the invention, the microfluidic system completely solves the technical problem such as microfluidic focusing, microfluidic driving and microfluidic detection. The designed sorting system can automatically sort different detected products. The designed fluidic control device can replace a plurality of existing microfluidic driving pumps. The solution promotes scientific researches on microfluidic chips and microfluidic driving technologies.

A method for producing a capsule for protein crystallization is provided. The method comprises adding a solution containing a protein and a gelling agent to an ionic cross-linking solution to form an ionically cross-linked gel capsule that encapsulates a solution of the protein.

An assay system designed to detect a protein biomarker in urine that is diagnostic for interstitial cystitis (IC). The presence of a 9 amino acid glycopeptide, antiproliferative factor (APF), in urine is unique to patients with IC. Urine samples from patients who exhibit symptoms consistent with IC are added to the assay system. Binding of APF to the cytoskeletal associated protein 4 (CKAP4) is positive for the presence of APF in urine and diagnostic for IC. The diagnostic system is a significant and surprising advance in diagnosis of IC and has commercial applications relevant to women and men who suffer from symptoms consistent with IC.

A bio-sensing device includes a first platform, a second platform, at least one first magnetic element, and at least one second magnetic element. The first platform is configured to support a microplate. The microplate has a plurality of wells, each of which stores a reagent and a plurality of microbeads. The second platform is movably placed below the first platform. The first and the second magnetic elements are respectively located on the second platform. The first magnetic element moves along with the second platform to magnetically attract the microbeads in the wells, the second magnetic element moves along with the second platform to demagnetize the microbeads in the wells, and magnetism of the first magnetic element is opposite to magnetism of the second magnetic element.

A real time analysis in accordance with temperature change and highly sensitive detection are permitted. A flow passage device has a flow passage (1) and is able to heat a fluid in the flow passage. A state of the fluid in the flow passage is observable using light. The flow passage device includes a first member (2) including an observation surface (3) and an upper inner wall surface (4), and a second member (5) including a lower inner wall surface that opposes the upper inner wall surface (4) and that is part of inner walls of the flow passage. A heating resistor (6) having a reflective surface that reflects light is provided on the lower inner wall surface. The light reflected by the reflective surface passes through the upper inner wall surface (4) and the observation surface (3).

The invention relates to phase-separated composite for microfluidic applications, whereby the polymerization /phase separation is performed in such a way that a top-layer of a certain ratio to the height of the composite is achieved in order to ensure the stability of the composite.

The invention relates to a processing method of a microfluidic electrical detection chip. The method comprises the following steps of drawing a three-dimensional model, drawing a three-dimensional model of the microfluidic electrical detection chip by using three-dimensional drawing software, and converting the three-dimensional model into an STL file; generating a printing path: generating the printing path after the three-dimensional model is sliced according to the STL file; printing a chip model, printing a sacrificial male mold used for manufacturing a micro-channel structure on a substrate, printing electrode structures with the same height as the sacrificial male mold on two sides of the sacrificial male mold, and printing a sealing structure used for covering the sacrificial male mold and the electrode structures above the sacrificial male mold and the electrode structures; and curing and molding a printing mold to obtain the integrated microfluidic electrical detection chip. Integrated manufacturing of the microfluidic electrical detection chip with the micro-channel structure and the detection electrode structures can be realized, and popularization and application of a microfluidic electrical detection technology in the fields of clinical instant diagnosis and the like are promoted.