8 results about "Shoot" patented technology

The invention discloses application of a gene GmRAV1 related to photoperiod adjusting and controlling of a soybean, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the soybean gene GmRAV1 is shown in SEQ ID NO.1, encoded protein is shown in SEQ ID NO.2. The soybean gene GmRAV1 can be used in processes of adjusting plant photoperiod sensitivity and promoting shoot regeneration in plants. By overexpressing the gene GmRAV1 of the soybean, shoot regeneration in plants is promoted; meanwhile, by silencing and interfering the expression of the gene GmRAV1 of the soybean, plant photoperiod sensitivity, plant height, bloom maturation period are changed, and the plant photoperiod sensitivity is lowered. The gene GmRAV1 of soybean can be applicable to cultivation and improvement of a new eurytopicity product with precocity and photoperiod insensitivity, solve the problem of flowering asynchronism in crossbreeding, breeding period controlling problem, photoperiod sensitivity problem and introduction problem of various crops, vegetables, fruit, and flowers.

The invention discloses a manufacture method of pyramidalis gardening quadripod, and belongs to the technical field of farm garden. The method comprises the steps of preparing a seedling bed, preparing shoot for cuttings, cutting, carrying out post-cutting management, selecting seedlings, planting for the first time, planting for the second time, planting for the third time, carrying out planting cultivation, designing a process size of the gardening quadripod, selecting a tree according to the quadripod, transplanting the tree, carrying out the post-planting management, facilitating growth of the tree, binding the tree by utilizing iron wires and a reinforcement net according to a designed pattern of each part, shaping and reinforcing four sides of the quadripod body, and carrying out the growth maintenance to obtain the pyramidalis gardening quadripod. By adopting the production method, the survival rate of the seedling is high, and the produced gardening quadripod is good in shape, is not deformed in the transplanting process, easy to maintain after the tree is transplanted and capable of facilitating the long-term growth of the tree.

The invention relates to a plant propagation technology, and particularly relates to a direct umbrellaleaf direct regeneration seedling-raising method based on a radical bud. The method comprises the following steps of: 1, treating an umbrellaleaf radical bud to be used as an explant for later use; 2, inducing and rooting multiple shoots of the umbrellaleaf radical bud, to be specific, adding 0.02-0.03mg.L<-1> of naphthylacetic acid, 0.15mg.L<-1> of 6-benayl aminopurine, 0.50-0.60mg.L<-1> of kinetin, 2.20-2.40mg.L<-1> of gibberellin in a basic culture medium; and 3, rapidly propagating a umbrellaleaf sprout. The direct umbrellaleaf regeneration seedling-raising method is used for rapidly propagating umbrellaleaf by using a mode of inducing the radical bud to directly regenerate the multiple shoots and rooting, and can be applied to factorized propagation of the umbrellaleaf sprout and the large-scale cultivation and development and utilization of the umbrellaleaf. The umbrellaleaf radical bud is highest in direct regeneration rate, and the average regeneration rate reaches above 100 percent; and the acclimatization and transplant survival rate reaches above 98.0 percent.

The invention relates to a preparation method of a shii-take culture medium with mulberry shoots being main raw materials, and belongs to the technical field of shii-take culture mediums. The mulberryshoots are placed into a smashing machine to be smashed, so that mulberry shoot wood bits are obtained; according to a formula proportion, 65-75 wt% of the obtained mulberry shoot wood bits, 20-30 wt% of rice bran, 0.5-2 wt% of calcium superphosphate, 1-3.4 wt% of lime, 0.1-1.5 wt% of urea and 0.5-1 wt% of white granulated sugar are mixed, so that a total ingredient is obtained, water with the mass being 1.2-1.5 times that of the total ingredient is added for uniform stirring, and then a culture material with pH being 6 is obtained; the obtained culture material is subjected to high-pressuresteam sterilization for 3-5 hours or normal-pressure steam sterilization for 12-24 hours, so that the shii-take culture medium is obtained. Spawn running time of the shii-take culture medium preparedthrough the method is short, and the biological efficiency of shii-take is high.