4 results about "Agrobacterium" patented technology

The invention relates to the field of molecular biology, and in particular to catalpa bungei CabuAP3 protein as well as an encoding gene and application thereof. The amino acid sequence of the catalpa bungei CabuAP3 protein is as shown in SEQ ID No.1; the sequence (CDS) of the encoding gene is as shown in SEQ ID No.2; the whole length of the cDNA sequence of the catalpa bungei CabuAP3 gene is as shown in SEQ ID No.3. The catalpa bungei CabuAP3 gene provided by the invention is only expressed in petals and stamens of catalpa bungei, and is not expressed in leaves, sepal or pistil. A CabuAP3 gene expression vector is transferred into mutants of wild arabidopsis and arabidopsis ap3-3, the result shows that the transgenic wild arabidopsis has one extra petal, which indicates that the CabuAP3 gene has the function of regulating and controlling increase of the number of petals of the transgenic wild arabidopsis, and the transgenic wild arabidopsis ap3-3 mutant has a filament structure. The plant variation material obtained from the catalpa bungei CabuAP3 gene expression vector can be applied to plant breeding and view admire, and has very good application prospect.

The invention discloses a genetic transformation method by the utilization of cotton meristematic tissue in the plant gene engineering field. According to the method, without the step of cotton tissue culturing, activated Agrobacterium containing target gene plasmid vector is directly dipped on a growing point removed cotton plant to induce adventitious bud to grow until the adventitious bud blossoms and grows bolls, and transgenic cells are screened by the use of antibiotics. By the utilization of the method for genetic transformation of cotton, differentiation rate is high, the screening method is simple, the cost for cotton genetic transformation is saved, and the time for cotton genetic transformation is shortened.

The invention discloses a rapid transgenosis method taking citrus seedlings planted in soil as materials. The rapid transgenosis method comprises the following steps: preparation of explants, preparation of agrobacterium infection liquid, wound infection of seedlings and molecular biological identification of buds to be transformed, wherein the preparation of the explants is that the seedlings planted in soil are directly taken as the explants to transform so as to obtain transgenic plants. According to the rapid transgenosis method disclosed by the invention, test-tube stock seedlings are not needed to be prepared, buds to be transformed are not needed to be adapted, and the seedlings planted in soil are directly wounded and transformed to obtain buds; and moreover, planting of seeds in soil in a greenhouse is not affected by the culminate and the weather, and can be performed all the year round. The rapid transgenosis method has the advantages of simple and convenient operation steps, low cost and short time.

Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.