21 results about "Mutation" patented technology

The invention discloses a serum specificity metabolite spectrum for a patient with a lung cancer and a building method thereof, and particularly discloses a method for carrying out liquid-phase/gas-phase chromatography mass spectrometry metabonomics analysis based on the patient suffering from the lung cancer. The method comprises the following steps: collecting fasting blood samples of a healthy and normal detected person and the patient suffering from the lung cancer 7 days before and after an operation, separating by a chromatogram column after processing; extracting and aligning original data of an instrument after adopting mass spectrometry analysis; building a mathematical model to analyze the metabolites spectrum by adopting multivariable data statistical software; comparing with the difference between an average person with the metabolites spectrum of the patient with the lung cancer, so as to determine the specificity metabolite spectrum. By adopting the method disclosed by the invention, the mutation status of the metabolite of the patient with the lung cancer can be generally and comprehensively reflected, and the serum specificity metabolite spectrum can be applied to early diagnosis of the lung cancer or a beneficial technical support is supplied for prognosis.

The present invention relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells carrying mutations in at least one of the effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells according to claim 1 carrying mutations in all effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to an expression vector for delivering a heterologous protein into a target cell using a Yersinia enterocolitica mutant strain according to any of the claims 1 to 4, which comprises in the 5' to 3' direction :(a) a promoter of a Yersinia virulon gene, (b) a first DNA sequence encoding a delivery signal from a Yersinia effector protein, operably linked to said promoter; and, (c) a second DNA sequence coding for said heterologous protein, fused in frame to the 3' end of said first DNA sequence. The present invention further relates to methods and compositions comprising (the use of) the afore-mentioned mutant strains and expression vectors.

The invention discloses a 1,3-1,4-beta-glucanase mutant, and belongs to the fields of gene engineering and enzyme engineering. Lysine in the 20th position, 117th position and 165th position of 1,3-1,4-beta-glucanase from Bacillus terquilensis mutates through an overlapping extension PCR method to form serine in order to obtain single mutants K20S, K117S and K165S respectively. The above three mutation sites are integrally mutated to obtain a K20S/K117S/K165S triple mutant enzyme. Four mutant enzymes have higher catalysis activity and better thermal stability. Compared with wild enzymes, the above mutant enzymes are in favor of realizing the industrial application.

The invention discloses a bdellovibrio bacteriovorus bacterial strain for eliminating listeria monocytogenes and an application thereof. The invention obtains bdellovibrio bacteriovorus BDSM08 by separation, purification and ultraviolet mutation. BDSM08 is in the arc-shaped unicellular form when observed by an electron microscope, the size thereof is 1.8*1.0 mu m, the ends thereof are provided with flagellum and the length of the flagellum is 3.2 mu m. The bdellovibrio bacteriovorus BDSM08 is cultivated with the two layer plating method at the temperature of 28 DEG C for three days to form transparent round negativecolony the diameter of which is 2-3mm and the optimum growing pH value of which is 7.2, the optimum growing temperature of which is 28 DEG C and the optimum salinity of which is0%. The microbial agent prepared by BDSM08 has a strong effect on killing listeria monocytogenes which can be commonly seen in environment. The invention applies the bdellovibrio bacteriovorus BDSM08for the first time to prevent and cure food listerellosis; the invention has a favourable effect on eliminating listeria monocytogenes on food, has characteristics of no toxicity, no side effect on food and the like, and has favourable application prospect.

The present invention relates generally to viral variants exhibiting reduced sensitivity to agents and in particular nucleoside analogues. More particularly, the present invention is directed to hepatitis B virus variants exhibiting complete or partial resistance to nucleoside analogues. The variants may also comprise corresponding mutations affecting immunological interactivity to viral surface components. The present invention further contemplates assays for detecting such viral variants which assays are useful in monitoring anti-viral therapeutic regimes and in developing new or modified vaccines directed against viral agents and in particular hepatitis B virus variants. The present invention also contemplates the use of the viral variants to screen for agents capable of inhibiting infection, replication and/or release of the virus.

The invention belongs to the plant vegetative propagation technology and particularly relates to a conical redpepper fruit vegetative propagation seedling method. The method comprises the steps that a seedbed with perlite serving as a substrate is established, a water spraying system capable of automatically spraying water mist is established, conical redpepper fruit branches serve as in vitro materials and are arranged in the seedbed after being processed with medicines, the water spraying system is started to meet the humidity requirement of a seedling environment, the conical redpepper fruit in vitro materials take roots and grow into seedlings quickly, and the seedling rate is high. Due to the fact that the conical redpepper fruit vegetative propagation seedling method belongs to vegetative propagation and is in a stable type in gene heredity, character mutation and variation cannot happen, and optimal maternal characters can be maintained better. The materials can be taken to perform rapid propagation at any time in a whole year, and there is no limit to use of raw materials and propagation time. The conical redpepper fruit vegetative propagation seedling method is suitable for factory-like, large-scale and intensive production of conical redpepper fruits.

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.

A GFP with an F64L mutation and an E222G mutation is provided. This GFP has a bigger Stokes shift compared to other GFPs making it very suitable for high throughput screening due to a better resolution. This GFP also has an excitation maximum between the yellow GFP and the cyan GFP allowing for cleaner band separation when used together with those GFPs.

The invention provides identification and application of pig CD4 gene function mutation site molecular breeding marker, and particularly provides four function mutation sites of CD4 gene. The four function mutation sites have impact on expression level, membrane localization and immune level of the CD4 gene. Four key mutation sites of the molecular marker are characterized by having mutation at basic groups of 193rd (A/G), 195th (G/C), 196th (A/G) and 202nd (C/G) of a CDS area. A new molecular marker is provided for marker assisted selection of pig immune characters.

Methods and compositions are provided for determining TMB in a tumor sample using transcriptome profiling data. Also provided herein are methods and compositions for determining the response of an individual with a specific TMB to a therapy such as immunotherapy.

The invention discloses an evolutionary optimization method for e-government affair performance assessment management. The evolutionary optimization method comprises the following steps: constructing a chromosome formalized random vector initial population of e-government affair performance assessment indexes; calculating a fitness function value according to the scoring completion condition, the importance weight and the special priority set values of the e-government affair performance assessment indexes; sequencing the chromosome vectors of the e-government affair performance assessment indexes according to the fitness function value, and judging whether the optimal chromosome vector meets an optimization target or not; if the optimal chromosome vector meets the optimization target, ending the algorithm; otherwise, generating a new chromosome vector through the mutation operation of the chromosome vector and the crossover operation between the two chromosome vectors, adding the new chromosome vector into the current population, and calculating the fitness function value of the new chromosome vector, sorting all chromosome vectors of the current population according to the fitness function value, and eliminating the last chromosome vector; judging whether the optimal chromosome vector meets an optimization target or not, and if the optimal chromosome vector meets the optimization target, ending the algorithm; otherwise, carrying out iterative evolutionary optimization again through the mutation operation and the crossover operation until the optimal chromosome vector meets the optimization target. The method can promote efficiency improvement and intelligence of e-government performance assessment management.

The present invention relates to plant genes involved in regulating flowering, and especially to genes involved in the induction of flowering in response to cold, or vernalization. In particular, the present invention provides the identification, cloning, and characterization of genes involved in vernalization, and specifically of VIP genes, as well as to the proteins encoded by these genes, and to methods of using the VIP genes and proteins. Mutants of VIP genes, where the mutation is a knock-out mutation, confer a vernalization independence, or constitutively vernalized, phenotype in a plant which in the non-mutant form requires vernalization to flower.

The invention relates to a DNA library for detecting and diagnosing corneal dystrophy disease-causing genes as well as application of the DNA library. The library comprises 24 disease-causing genes related to corneal dystrophy; 24 corneal dystrophy disease-causing genes are preferably selected, a probe pool is designed, a target area library aiming at the 24 corneal dystrophy disease-causing genesis established, and the library performs sequencing by a high-throughput sequencing technology, looks for disease-cause mutation and provides genetic and molecular biological basis for clinical diagnosis. The library has the characteristics of accuracy, rapidness, flexibility and low cost; 24 gene detection areas can detect various kinds of common corneal dystrophy such as epithelial and subepithelial corneal dystrophy, Bowman's membrane corneal dystrophy, stromal corneal dystrophy, descemet's membrane and corneal endothelium dystrophy; and important significance and clinical value in diagnosis and differential diagnosis on the corneal dystrophy are achieved.