6 results about "Multiplex" patented technology

The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection/readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

The invention discloses a method for synchronously detecting five important virus pathogens in ranunculus asiaticus. The five important virus pathogens are ranunculus mild mosaic virus, ranunculus latent virus, ranunculus asiaticus white mottle virus, ranunculus asiaticus mosaic virus and ranunculus leaf distortion virus. According to the application of the method, plant total RNA or virus RNA isextracted, different types of virus cDNA first chains are synthesized by random primers, five viruses are subjected to multiplex PCR detection by specific primers, the steps of synthesizing the cDNA first chains by reverse transcription of a single virus primer, determining and analyzing a virus nucleotide sequence and the like are omitted, a target fragment is obtained by utilizing the rapidity and sensitivity of multiplex PCR, and a scientific method is established for detecting various ranunculus asiaticus virus disease samples quickly, accurately and sensitively in quantity.

The invention discloses a kit for identifying MCR genotyping, identification method and application of the kit The kit comprises a primer pair shown as SEQ ID No: 1 and SEQ ID No: 2, a probe shown asSEQ ID No: 3, a primer pair shown as SEQ ID No: 4 and SEQ ID No: 5, a probe shown as SEQ ID No: 6, a primer pair shown as SEQ ID No: 7 and SEQ ID No: 8, a probe shown as SEQ ID No: 9, a primer pair shown as SEQ ID No: 7 and SEQ ID No: 10 and a probe shown as SEQ ID No:11. After the mcr8 and mcr10 specific primer pairs adopt a unified forward primer sequence SEQ ID No: 7, and specific fragments without mutual interference are selected to design the specific fluorescent probe, so that a multiple PCR primer system is simplified to achieve a good amplification effect, and typing of mcr8 and mcr10is completed with high specificity; and the blank that a multiplex PCR kit for realizing effective typing and detection of all MCR genes in a clinical sample does not exist on the market at present isfilled up, and particularly the blank of the multiplex PCR kit capable of effectively identifying the functions of typing mcr8 and mcr10 is filled up.