Method for synchronously detecting five important virus pathogens in ranunculus asiaticus

A technology of simultaneous detection and ranunculus, applied in the field of plant virology, can solve the problem of unclear occurrence of diseases and insect pests, and achieve the effect of sensitivity advantage and accuracy advantage.

Active Publication Date: 2020-12-04
云南省农业科学院生物技术与种质资源研究所
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Benefits of technology

This patented technology allows for quick identification of diseases caused by these three classes of organisms found on plants called Lonomiaceae family that are spreading worldwide through rainwater floods. It also includes methods used to identify other important crops such as rice without being limited only one type of virus at once. These techniques help researches improve understanding about how they work together with each others' genetic material.

Problems solved by technology

The technical problem addressed in this patented text relates to preventing diseased vegetables caused by harmful fungi called raspberries, while maintaining their healthiness during harvesting processes due to its ability to transmit germplasm causing severe damages on crop products grown throughout the country's growing areas.

Method used

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  • Method for synchronously detecting five important virus pathogens in ranunculus asiaticus

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with the examples, but the present invention is not limited in any way, and any transformation or substitution made based on the teaching of the present invention belongs to the protection scope of the present invention.

[0030] The five important viral pathogens described in the present invention are ranunculus light mosaic virus, ranunculus recessive virus, ranunculus white mottle virus, ranunculus mosaic virus and ranunculus leaf shrinkage virus, characterized in that the The method for the simultaneous detection of five important viral pathogens in ranunculus comprises the following steps:

[0031] Extract the total plant RNA or viral RNA in the sample to be tested;

[0032] Specific primers designed to amplify gene fragments of viral pathogens ranunculus light mosaic virus, ranunculus recessive virus, ranunculus white spot virus, ranunculus mosaic virus, ranunculus leaf shrinkage virus;

[0033] Sy

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Abstract

The invention discloses a method for synchronously detecting five important virus pathogens in ranunculus asiaticus. The five important virus pathogens are ranunculus mild mosaic virus, ranunculus latent virus, ranunculus asiaticus white mottle virus, ranunculus asiaticus mosaic virus and ranunculus leaf distortion virus. According to the application of the method, plant total RNA or virus RNA isextracted, different types of virus cDNA first chains are synthesized by random primers, five viruses are subjected to multiplex PCR detection by specific primers, the steps of synthesizing the cDNA first chains by reverse transcription of a single virus primer, determining and analyzing a virus nucleotide sequence and the like are omitted, a target fragment is obtained by utilizing the rapidity and sensitivity of multiplex PCR, and a scientific method is established for detecting various ranunculus asiaticus virus disease samples quickly, accurately and sensitively in quantity.

Description

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Claims

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Application Information

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Owner 云南省农业科学院生物技术与种质资源研究所
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