1 results about "Mutation breeding" patented technology
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Mutation breeding, sometimes referred to as "variation breeding", is the process of exposing seeds to chemicals or radiation in order to generate mutants with desirable traits to be bred with other cultivars. Plants created using mutagenesis are sometimes called mutagenic plants or mutagenic seeds. From 1930 to 2014 more than 3200 mutagenic plant varieties were released that have been derived either as direct mutants (70%) or from their progeny (30%). Crop plants account for 75% of released mutagenic species with the remaining 25% ornamentals or decorative plants. However, although the FAO/IAEA reported in 2014 that over 1,000 mutant varieties of major staple crops were being grown worldwide, it is unclear how many of these varieties are currently used in agriculture or horticulture around the world, as these seeds are not always identified or labeled as having a mutagenic provenance.
The invention discloses enterobacter cloacae for stably producing 2,3-butanediol at high yield and a method for compound mutation by using low-temperature plasma and diethyl sulfate. The enterobacter cloacae is classified and named as Enterobacter cloacae DLM and has the preservation number of CGMCC (China General Microbiological Culture Collection Center) 6053. The mutation method is characterized in that bacterium is suspended into physiological saline and pretreatment of diethyl sulfate and compound mutation of the low-temperature plasma and the diethyl sulfate are sequentially carried out. The strain can be used for producing the 2,3-butanediol by fermenting different effectively-utilized carbon sources, wherein the conversion rate of sugar is high and the concentration of the 2,3-butanediol is high; and when glucose or synanthrin is hydrolyzed into a carbon source, the concentrations of the 2,3-butanediol in a 5L fermentation tank respectively reach 125.2g / l and 120.2g / l. The mutation method used in the invention has the characteristics of simpleness and feasibility in operation, short treatment time in mutation and the like and provides a reliable method for mutation breeding of microorganisms.