1 results about "Diethyl sulfate" patented technology
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Diethyl sulfate is a highly toxic and likely carcinogenic chemical compound with formula (C₂H₅)₂SO₄. It occurs as a colorless, oily liquid with a faint peppermint odor and is corrosive. Diethyl sulfate is used as an alkylating agent to prepare ethyl derivatives of phenols, amines, and thiols. It is used to manufacture dyes and textiles.
The invention discloses enterobacter cloacae for stably producing 2,3-butanediol at high yield and a method for compound mutation by using low-temperature plasma and diethyl sulfate. The enterobacter cloacae is classified and named as Enterobacter cloacae DLM and has the preservation number of CGMCC (China General Microbiological Culture Collection Center) 6053. The mutation method is characterized in that bacterium is suspended into physiological saline and pretreatment of diethyl sulfate and compound mutation of the low-temperature plasma and the diethyl sulfate are sequentially carried out. The strain can be used for producing the 2,3-butanediol by fermenting different effectively-utilized carbon sources, wherein the conversion rate of sugar is high and the concentration of the 2,3-butanediol is high; and when glucose or synanthrin is hydrolyzed into a carbon source, the concentrations of the 2,3-butanediol in a 5L fermentation tank respectively reach 125.2g / l and 120.2g / l. The mutation method used in the invention has the characteristics of simpleness and feasibility in operation, short treatment time in mutation and the like and provides a reliable method for mutation breeding of microorganisms.