The present invention discloses a method for scoring and sequencing the neoantigens immunological activity based on eigenvalues of tumor neoantigens. The method comprises the following steps of: inputof WGS / WES and
RNA-seq
sequencing data of tumor-normal samples, prediction and
annotation of tumor cellc
mutation, and calculation of related eigenvalues; extraction of neoantigens related eigenvalues; setting of a scoring function of the neoantigen activity; and neoantigen sequencing based on the scoring function of the neoantigen activity. According to the method disclosed by the present invention, the tumor
cell mutation is analyzed and calculated, and the
mutation annotation is completed to calculate the partial eigenvalue, and the MHC-I binding neoantigen is predicted to calculate the partial eigenvalue; all eigenvalues related to the tumor neoantigens are extracted so as to set the scoring function of the neoantigen activity, and finally through the scoring function of the neoantigen activity, the neoantigens are sequenced. Compared with the traditional
screening method, the method is more efficient and precise, and has important application value for tumor
immunotherapy.