Callery pear ascorbate peroxidase gene and use thereof in resisting heavy metal stress,Catalpa bungei CabuAP3 protein as well as encoding gene and application thereof

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2 results about "Arabidopsis" patented technology

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Arabidopsis (rockcress) is a genus in the family Brassicaceae. They are small flowering plants related to cabbage and mustard. This genus is of great interest since it contains thale cress (Arabidopsis thaliana), one of the model organisms used for studying plant biology and the first plant to have its entire genome sequenced. Changes in thale cress are easily observed, making it a very useful model.

Callery pear ascorbate peroxidase gene and use thereof in resisting heavy metal stress

InactiveCN109295077ABacteriaOxidoreductasesEscherichia coliHeterologous

The invention relates to a callery pear ascorbate peroxidase gene and a use thereof in resisting heavy metal stress. The gene has a nucleotide sequence shown in the sequence SEQ ID No. 1. The total RNAs of the leaves of the callery pear are extracted after cadmium treatment and the ascorbate peroxidase gene Pc. APX is cloned by combination of bioinformatics and PCR so that the complete coding genesequence of 753 bp is obtained. The escherichia coli expression vector pET-22b(+)-Pc. APX is constructed and through the escherichia coli heterologous expression system, the functions of the cloned ascorbate peroxidase gene Pc. APX are identified and the recombinant escherichia coli with the cloned ascorbate peroxidase genes Pc. APX have strong tolerance to cadmium. At the same time, a binary plant expression vector pRI201-AN-GUS-Pc. APX is contructed, the vector is transferred into agrobacterium tumefaciens GV3101 cells by a freezing-thawing method, and the cells are transformed into Arabidopsis thaliana, and the obtained transgenic Arabidopsis thaliana has higher cadmium tolerance than wild-type Arabidopsis thaliana.

Owner:JIANGSU ACAD OF AGRI SCI

Catalpa bungei CabuAP3 protein as well as encoding gene and application thereof

InactiveCN105111291ABacteriaPlant peptidesAgricultural scienceWild type

The invention relates to the field of molecular biology, and in particular to catalpa bungei CabuAP3 protein as well as an encoding gene and application thereof. The amino acid sequence of the catalpa bungei CabuAP3 protein is as shown in SEQ ID No.1; the sequence (CDS) of the encoding gene is as shown in SEQ ID No.2; the whole length of the cDNA sequence of the catalpa bungei CabuAP3 gene is as shown in SEQ ID No.3. The catalpa bungei CabuAP3 gene provided by the invention is only expressed in petals and stamens of catalpa bungei, and is not expressed in leaves, sepal or pistil. A CabuAP3 gene expression vector is transferred into mutants of wild arabidopsis and arabidopsis ap3-3, the result shows that the transgenic wild arabidopsis has one extra petal, which indicates that the CabuAP3 gene has the function of regulating and controlling increase of the number of petals of the transgenic wild arabidopsis, and the transgenic wild arabidopsis ap3-3 mutant has a filament structure. The plant variation material obtained from the catalpa bungei CabuAP3 gene expression vector can be applied to plant breeding and view admire, and has very good application prospect.

Catalpa bungei CabuAP3 protein as well as encoding gene and application thereof

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Owner:INST OF FORESTRY CHINESE ACAD OF FORESTRY

2 results about "Arabidopsis" patented technology

Callery pear ascorbate peroxidase gene and use thereof in resisting heavy metal stress

Catalpa bungei CabuAP3 protein as well as encoding gene and application thereof

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