3 results about "Fluorescent protein" patented technology

The present invention discloses a method for labeling specific cells within living cells or tissues. The method comprises preparing the vectors with genes of photoactivable fluorescent proteins, followed by injecting the vectors containing genes of photoactivable fluorescent proteins together with genes of other fluorescent proteins into living cells or tissues, resulting in biological tissues with traceable systemic expression and irradiating the predetermined areas in living cells or tissues with an activating light source, thereby enhancing the intensity and duration of the emitted fluorescent after other excitations, thus revealing the targets intended for observation out of the background, so that a target-oriented image tracing is achieved.

The invention provides a CEA diagnosis kit based on a bimolecular fluorescence complementation technology, wherein the kit comprises an anti-CEA antibody conjugated fluorescent protein N-terminal fragment and an anti-CEA antibody conjugated fluorescent protein C-terminal fragment. The invention further discloses a preparation method of the CEA diagnosis kit, wherein the preparation method comprises: preparation of an anti-CEA antibody conjugated fluorescent protein N-terminal fragment, and preparation of an anti-CEA antibody conjugated fluorescent protein C-terminal fragment. The invention further discloses a use method the kit. According to the present invention, the kit has advantages of simple operation, no cleaning, high precision, high accuracy and the like, is conveniently suitable for clinical detection, can increase the accuracy of the diagnosis of tumors in the monitoring of tumors, and has great market value.