The invention provides a CEA diagnosis kit based on a
bimolecular fluorescence complementation technology, wherein the kit comprises an anti-
CEA antibody conjugated
fluorescent protein N-terminal fragment and an anti-
CEA antibody conjugated
fluorescent protein C-terminal fragment. The invention further discloses a preparation method of the CEA diagnosis kit, wherein the preparation method comprises: preparation of an anti-
CEA antibody conjugated
fluorescent protein N-terminal fragment, and preparation of an anti-CEA
antibody conjugated fluorescent
protein C-terminal fragment. The invention further discloses a use method the kit. According to the present invention, the kit has advantages of simple operation, no cleaning, high precision, high accuracy and the like, is conveniently suitable for clinical detection, can increase the accuracy of the diagnosis of tumors in the monitoring of tumors, and has great market value.