Method for labeling specific cells within living cells or tissues
a technology for living cells and tissues, applied in biochemistry apparatus and processes, peptides, genetic material ingredients, etc., can solve the problems of long life span of transgenic mammals, ineffective use of inability to effectively combine proteins and living tissues, etc., to facilitate the exploration of influences and variations.
Inactive Publication Date: 2008-05-29
NATIONAL TSING HUA UNIVERSITY
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Benefits of technology
[0013]One advantage of the present invention is to provide a method for tracking transgenic organisms of, including but not limited to, Drosophila, and providing the transgenic Drosophila line with target genes to facilitate the exploration for influences and variations induced by target genes.
[0014]Another advantage of the present invention is to provide a method for labeling specific cells within transgenic cells or tissues of, including but not limited to, Drosophila. The genes of green fluorescence protein (GFP) are inserted into Drosophila embryos as the label by transgenic technology.
[0015]Still another advantage of the present invention is to provide a method for studying transgenic Drosophila through exploring the intracellular protein dynamics, movements and pathway by tracking the visible green fluorescence protein (GFP) within the cell.
[0016]Additional advantage of the present invention is to provide a method for labeling specific cells in transgenic Drosophila. The proteins within the living cells or tissues are visible due to the combination of green fluorescence protein (GFP) and homologues fluorescence protein. The present invention also provides the information regarding the intracellular protein movements and protein-protein interactions.
[0017]One object of the present invention is to disclose a method for labeling specific cells in living brain of transgenic Drosophila.
[0018]Another object of the present invention is to disclose a method for tracking the development of a target neuron and other connections among other neurons in transgenic Drosophila. The present invention is also used for labeling the establishment of any neural circuit in transgenic Drosophila brain.
Problems solved by technology
However, a pending problem is how to observe effectively gene distributions with particular functions within tissues or organs.
However, no effective tool exists for labeling specific cells within the tissues and organs.
However, these proteins have not yet been combined with living tissues effectively.
However, a principal obstacle in performing gene / disease analysis on transgenic mammals is the long life span of animals.
However, when using this methodology, as in all prior methods, individual neurons within the whole neural circuitry in the fly brain cannot be selectively analyzed.
Method used
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Embodiment Construction
[0026]The drawings and the following descriptions present and describe the purpose of illustrating the preferred embodiments of the present invention only, and are not for the purpose of limiting the present invention. The present invention provides the information for intracellular protein expression and neuron networks, and a preferred embodiment for protein expressions induced in Drosophila embryos. Such an expressing system should be modular to facilitate expansion for multiple proteins with different functions.
[0027]Several photoactivable proteins are disclosed. For instance, photoactivable green fluorescent protein (PA-GFP) (Science (297):1873, 2002), kindling fluorescent protein 1 (KFP1) (Nat Biotechnol. (21):192, 2003) and Kaede (PNAS, 2002) have been developed in recent studies.
[0028]The photoactivable protein introduced facilitates precise photolabeling and tracking of the protein, thereby, providing complete information of the protein's dynamics. The photoactivable protei...
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The present invention discloses a method for labeling specific cells within living cells or tissues. The method comprises preparing the vectors with genes of photoactivable fluorescent proteins, followed by injecting the vectors containing genes of photoactivable fluorescent proteins together with genes of other fluorescent proteins into living cells or tissues, resulting in biological tissues with traceable systemic expression and irradiating the predetermined areas in living cells or tissues with an activating light source, thereby enhancing the intensity and duration of the emitted fluorescent after other excitations, thus revealing the targets intended for observation out of the background, so that a target-oriented image tracing is achieved.
Description
FIELD OF THE INVENTION[0001]The present invention relates to a method for labeling living cells or tissues, and more specifically, to provide a method for observing or labeling specific cells within living cells or tissues with fluorescent proteins in transgenic preparations.BACKGROUND OF THE INVENTION[0002]Biotechnology has had made remarkable contributions to the development of medical science rapidly. Some new inventions are already widely applied in biochemical engineering, cell culture culturing and materials science. The recent accomplishment of mapping the human genome, along with the development of bioinformatics, nanotechnology and proteomics, will eventually help us comprehend the mechanisms of genetic processes and diseases, and let us identify differences among individual humans, and the reasons why a certain therapy fails to work for someone. The ultimate objective of these researches is always to resolve the problems associated with human health. However, a pending pro...
Claims
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IPC IPC(8): C12N15/87A61K31/7052
CPCA01K67/0339C12N15/85C07K14/43595A61K31/7052
Inventor CHIANG, ANN-SHYNFU, TSAI-FENG
Owner NATIONAL TSING HUA UNIVERSITY
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