Fluorescence detecting system for quantitative gene magnification instrument
A technology for fluorescence detection and gene amplification, applied in the field of fluorescence detection systems, can solve the problems of complex metals, large scattering and attenuation of light intensity, and high cost, and achieve the effects of reducing manufacturing costs and improving detection sensitivity.
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[0010] Quantitative gene amplification instruments are generally composed of a fluorescence detection system, a data processing system and a display system. The fluorescence detection systems of the existing quantitative gene amplification instruments are such as figure 1 1-1 is the excitation light, 1-2 is the fluorescence emission, 1-3 is the sample, 1-4 is the sample base, 1-5 is the cap scattered light, 1-6 is the cap, 1- 7 is the liquid surface scattered light, 1-8 is the sample liquid level. When the excitation light is reflected by the sample and reaches the interface 1-8 between the sample liquid and the air, part of the light scattering 1-7 occurs, and then when the reflected light reaches the cap 1-6, light scattering 1-5 occurs again, thus The intensity of light is greatly weakened, which affects the sensitivity of detection.
[0011] The quantitative gene amplification instrument of the present invention is also composed of a fluorescence detection system, a data pro
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