4 results about "Porcine circovirus" patented technology

The invention discloses a chemiluminescence immunoassay kit for Seneca valley virus nonstructural protein 3ABC antibody detection. The kit comprises a Seneca valley virus nonstructural protein 3ABC antigen coated chemiluminescence immunoassay plate, positive control serum, negative control serum, an enzyme-labeled antibody, a sample diluent, a chemiluminescence substrate solution, a luminescence enhancing agent and concentrated washing liquid, wherein the amino acid sequence of the Seneca valley virus nonstructural protein 3ABC is shown as SEQ ID NO.2. The kit provided by the invention uses nonstructural protein 3ABC antigen expressed by a prokaryotic expression system for coating a reaction plate; the antigen consumption is low; whether the Seneca valley virus exists in the pig serum or not can be efficiently detected; in addition, reaction with the swine vesicular disease viruses, hog cholera viruses and porcine circovirus type 2 cannot occur. An enzymatic chemiluminescence reactionsystem is used for judging the result; the detection sensitivity is improved. The kit provided by the invention has the advantages that the specificity is high; sensitivity and high efficiency are realized; good market prospects are realized.

The invention provides PCV2-PRV (porcine circovirus type 2-porcine pseudorabies virus) two-temperature type double PCR (polymerase chain reaction) detection primers and a diagnosis method thereof. The primers comprise a PCV2 primer pair and a PRV primer pair, wherein the PCV2 primer pair comprises an upstream primer PCV2-F1 and a downstream primer PCV2-F2; the PRV primer pair comprises an upstream primer PRV-F3 and a downstream primer PRV-F4. Key parameters of the two-temperature type PCR are explored, so that PCV2 and PRV can be detected synchronously, and the primers are simple to operate, low in technological requirement, low in cost, time-saving and labor-saving, have good specificity, sensitivity and stability and are suitable for clinical popularization and application.

Vaccine compositions and methods are described for providing immunity to porcine circovirus type two (PCV2) genotypes including by administration of a recombinant PCV2 capsid polypeptide which comprises antigenic epitopes from the capsids of multiple PCV2 genotypes. In other embodiments a recombinant chimeric porcine circovirus is provides for use as a vaccine that combines the nonpathogenic backbone of porcine circovirus type 1 (PCV1) with the sequences encoding a PCV2 capsid polypeptide comprises antigenic epitopes from the capsids of multiple PCV2 genotypes.