23 results about "Organism" patented technology

The present invention relates to methods of treating, preventing, or lessening the severity of resistant bacterial infections in mammals, utilizing compounds of formula I or formula VII or pharmaceutically salts thereof. The present invention also relates to methods of using compounds of formula I or formula VII in combination with one or more additional antibacterial agents and/or one or more additional therapeutic agents that increase the susceptibility of bacterial organisms to antibiotics.

The invention discloses denitrifying phosphorus removal bacteria bacillus cereus H-hrb01, and belongs to the technical fields of biological engineering and environmental engineering, wherein bacillus cereus H-hrb01 belongs to bacillus genus by identification and is conserved in the general microbiology centre of the China committee for culture collection of microorganisms, the conservation date is March 27th 2012, and the conservation number is CGMCC NO.5939; bacillus cereus H-hrb01 can take various organic matters as carbon sources, has good denitrifying and phosphorus-removing efficiency, and can be used for sewage biological denitrification and dephosphorization process; bacillus cereus H-hrb01 is activated and cultivated, and fed into artificial wastewater to mix and then introduced into an SBR (styrene butadiene rubber) biofortification device, and each effluent index of the reactor is superior to that of a contrast system which is not fed with the bacterial strain. The removal rates of the COD (chemical oxygen demand), ammonia nitrogen and dissoluble phosphorus in the sewage are respectively up to 93.90%, 97.70% and 96.25%.

The invention provides a selenium-enriched mushroom grass culture medium which is prepared by taking selenium-enriched mushroom grass, wood chips, wheat bran, saccharose, gypsum, monopotassium phosphate and magnesium sulfate as the main raw materials. The invention further provides a method for producing shiitake mushrooms through the selenium-enriched mushroom grass culture medium. Accordingly, a traditional shiitake mushroom producing method is changed, a selenium-enriched mushroom grass technology is utilized in shiitake mushroom stick production, therefore, the producing method of selenium-enriched products is changed, the nutritional value of the shiitake mushrooms is increased, the production efficiency is improved, the shiitake mushroom yield and biotransformation rate are high, and meanwhile the production cost is greatly lowered; in addition, the producing method is simple in technology, the produced shiitake mushrooms are high in content of selenium-enriched elements, the product is free of pollution, and the food safety problem is solved from the source.

Present invention relates to the Biomarkers for detecting high altitude adaptation and hypoxia responsiveness and the method thereof. The invention specifically relates to the Gene variants SNPIDs rs479200 and rs480902 in the first intron of EGLN1 (Prolyl Hydroxylase 2) gene as biomarkers for adaptation to high altitude and predisposition for high altitude pulmonary edema and hypoxia responsiveness using a novel integrative approach of phenotyping concepts of Ayurveda with population genetics, and disease genomics. More specifically, the C allele of SNP ID rs480902 and T allele of rs479200 of EGLN1 gene is more frequent in patients of HAPE and nearly absent in native highlanders. The present invention also provides primers and methods suitable for the detection of these allelic variants for the prediction of individual's adaptability to high altitude and hypoxia and/or the genetic analysis of the EGLN1 gene in a population.

An apparatus for acclimating an aquatic organism, contained in a partially water filled plastic bag, to an environment in an aquarium includes an aquarium frame comprising a first portion exterior to the aquarium and a second portion interior to the aquarium. A bag holder is operable to hold a top of the plastic bag in an open position. The bag holder is positioned on the second portion where the top of the plastic bag is above a top level of water in the aquarium and a substantial portion of the plastic bag is below the top level. A dripping cup is operable to release water obtained from the aquarium into the plastic bag. The dripping cup is joinable to the first portion, whereby the aquatic organism is acclimated to a chemistry and temperature of the water in the aquarium.

The present application relates to methods for determining the occurrence of a liver disease in a subject by particular polypeptides biomarkers, and to kits using such biomarkers.

The invention specifically relates to a method for estimating in-vitro detoxification effect of a mycotoxin detoxification agent by using liquid chromatography, belonging to the technical field of biological detection. The method comprises the following steps: (1) early stage preparation: I, drafting of a standard curve of mycotoxin; II, preparation of a sample of the detoxification agent; and III, acquisition of a reaction medium; (2) detoxification: I, evaluation of in-vitro adsorption detoxification; and II, evaluation of simulated in-vivo adsorption detoxification; and (3) liquid chromatographic detection. The method for estimating in-vitro detoxification effect of the mycotoxin detoxification agent by using liquid chromatography can simulate gastrointestinal environments, adopts supernatant of chyme in the alimentary canal of an animal as a reaction medium, completely reproduce the gastrointestinal environment in the animal and more authentically evaluate the adsorption effect of an adsorbent.

Antibodies which specifically bind to components of the DNA synthesome which are altered in malignant cells are disclosed. These antibodies can be used, inter alia, to diagnose, prognoses, and treat malignancy and in assays to screen cells, tissues, and body fluids for the presence of a malignant phenotype. These antibodies can be further used to identify test compounds having the ability to suppress the malignant phenotype in a cell by assaying for the ability to inhibit or block the function of an altered component of the DNA synthesome associated with the malignant phenotype. Further, disclosed herein are methods and kit for minimally invasively detecting the presence of neoplasms and malignant conditions using easily obtainable body fluids, such as blood, plasma, lymph, pleural fluid, spinal fluid, saliva, sputum, urine, and semen, for example, to both detect the presence of cancer as well as assess the stage of the disease and the prognosis of the patient. By detecting the presence of an altered form of a component of the DNA synthesome in body fluid, one can diagnose and prognose malignancy. The disclosed method and kit therefor can be used as a diagnostic biomarker for malignancy as well as a means of monitoring the progress and effectiveness of therapeutics.

The invention belongs to the technical field of pest control and particularly relates to a comprehensive control method for wax-moth larvae in bee hives. The method has the advantages various plant components are mixed and crushed according to a specific proportion, bamboo vinegar is added to perform constant-temperature water batch extraction to obtain a green insecticide, the green insecticide is applied to the bee hives and honeycombs through a spraying method and a fumigating method, the honeycombs are taken out, independently sealed and frozen under a sealed and oxygen-free condition, thekilling efficiency of the wax-moth larvae can be increased greatly, and wax-moth larva incidence rate, wax-moth larva number of each honeycomb and wax-moth larva egg number in each honeycomb are lowered evidently; biological control and physical control are combined, the wax-moth larvae can be controlled in an efficient and high-quality manner, the method is safe to the environments and other organisms, residual pollution is avoided, and the original state and quality of honey can be guaranteed.

The invention discloses antioxidant polypeptide expression pichia pastoris engineering bacteria and application. According to the antioxidant polypeptide expression pichia pastoris engineering bacteria and application, the genetic engineering technology is used to build and obtain antioxidant polypeptide expression pichia pastoris engineering bacteria KM71H FZM2014 AOP, CCTCC NO: M 2014552 which can achieve high expression of antioxidant polypeptide VECYGPNRPQF. The prepared antioxidant polypeptide can efficiently remove free radicals and show potent biological activity in an animal experiment with weaned piglets serving as a model. The antioxidant polypeptide has the advantages of being feasible in preparation method, easy to operate, high in expression level, low in cost, high in biological activity and the like and can be widely used in industries, such as breeding, food, health products, aquatic products, infants and cosmetics.

The invention provides a solution for evaluating the glucose metabolism based on the sialology method and an evaluation method thereof. The solution comprises the following components: 60-150 mM of pH 7.6 Tris-HCl, 0.12-0.4% of blue chromogenic substances; 1200-2500 IU/L of glucose oxidase; 120-250 IU/L of horse radish peroxidase; and 600-1500 IU/L of antioxidant. The evaluation method comprises the steps of 1) collecting the saliva; 2) mixing the saliva with the above solution; 3) determining the level of glucose metabolism of an organism according to the color depth of the mixed solution of the saliva and the solution. According to the technical scheme of the invention, the level of glucose metabolism is evaluated by adopting the above solution that can change in color depth after reacting with the saliva. In this way, the inconvenience caused by the detection of the blood glucose can be solved. Meanwhile, the evaluation method is visual, convenient, fast, high in efficiency and high in accuracy. At the same time, the antioxidant is added into the above solution, so that the storage life of the solution is prolonged. Moreover, the waste is avoided and the cost is saved.

Described herein are RNA-based antisense therapeutics to target antibiotic resistant bacteria. The antisense therapeutics disclosed herein can be useful in re-sensitizing drug-resistant bacteria to antibiotics, as well as developing antibiotics that have bactericidal or bacteriostatic effects on the drug-resistant bacteria or are capable of preventing emergence of antibiotic resistance. Also described herein are methods for re-sensitizing a subject to one or more antibiotics in need thereof, methods for identifying target genes involved in adaptive antibiotic resistance in bacteria, and methods for developing antibacterial antisense therapeutics.

The present invention provides a method for systematically controlling rapid proliferation of cyanobacteria cells in lakes in spring. The method applies physical, biological and chemical methods in an integrated and synergistic manner and includes using flexible enclosures to close a control water area and using modified soil to adsorb and settle algal cells; coupling four technologies including algae control by fish, algae control by biological floating islands, algae inhibition by plants and algae control by microorganisms; and using H₂O₂ to kill algae and using 40% FeCl₃+CaCO₃ and 50% Ca(NO₃)₂ to treat sediment. The method of the present invention has established a complete system for physical, chemical and biological control of algae, forms joint control of exogenous contaminants, endogenous contaminants and cyanobacteria, integrates physical, biological and chemical methods and optimizes systematic methods that are used synergistically.

An abalone mushroom cultivation technology comprises the steps of 1, mechanical mixing and bagging; 2, separate and centralized sterilization; 3, three-level purification and inoculation; 4, intelligent mushroom house cultivation; 5, abalone mushroom house fruiting stage fertility management. The optimal formula and fruiting management control mode suitable for abalone mushroom cultivation are adopted, a foundation is laid for stable and high yield, the biological efficiency of finished mushroom is 55% or above, mushroom is round and has a good form, selling price is effectively increased by 20% or above, sustained and efficient market supply is ensured, and comprehensive benefits are increased by 25% or above.

The invention relates to the technical field of biological 3D printing, in particular to a method for facilitating in-vitro maturation of human immature oocytes by utilizing a 3D printing technology. The method comprises the following steps of (1) properly treating naked ova and granular cells of ovary cells; (2) performing in-vitro balanced culture of ovarian granular cells; (3) putting oocytes into the ovarian granular cells, and continuing to perform culture for 2 days; (4) preparing 3D printing ink; (5) preparing 3D printing gel; and (6) printing out follicles by adopting the 3D printing technology, putting the oocytes and an in-vitro maturation culture solution into the follicles, and performing culture for one week. According to the method, the follicles with a biochemical environment similar to that of a human body try to be simulated, and then the oocytes are quickly and accurately put into the follicles for performing artificial culture by depending on the 3D printing technology, so that the in-vitro maturation of the human immature oocytes is realized; the method depends on the 3D printing technology; and the whole process is quick and convenient, and the automation degree is high, so that the technical expense of the in-vitro maturation of the oocytes can be reduced.

The invention provides a method for propagating the trichogramma pintoi voegele indoors by the grapholitha molesta busck egg. The method comprises the steps of: taking the grapholitha molesta busck egg as the host of a parasite, and continuously propagating the trichogramma pintoi voegele generation by generation under the laboratory conditions. The grapholitha molesta busck egg is taken as the host of the parasite, the artificial propagation of the trichogramma pintoi voegele is not limited by the time and the space and can be continuously propagated by means of subculture, the trichogramma pintoi voegele propagating quality is high, the parasitic rate is stable, and the method has various advantages, so that the method offers a new approach for preventing the trichogramma pintoi voegelefrom being propagated in quantity in the field of the biological prevention of the farming and forestry pests.

The invention provides a method for cultivating cruciferae plants for observing the plasmodiophoromycetes infection. Root organizations of the initially-pathogenetic cruciferae plants are taken to prepare a plasmodiophoromycetes solution, cruciferae seeds are sowed in quartz sand, and the quartz sand and the seeds are soaked with an MS basic culture solution; under the conditions that the illumination intensity is 100 micro-mol photons/(m<2>.s), the illumination time ranges from 16 h to 18 h, the temperature ranges from 20 DEG C to 22 DEG C, and the humidity is higher than 75%, the seeds are cultivated until seed germination and are grown into plants with two main leaves. The plasmodiophoromycetes solution is applied to the roots of the plants to conduct the infection, and then root systems of the infected plants are observed through a biological microscope in a grading mode until the infection stage is finished. The method is easy and convenient to operate, distinct in observation result, small in occupied space and low in test cost, and has the advantages of being safe in operation, simple, high in repeatability, convenient to observe and distinct in result.

The invention discloses a method for detecting infectious pathogens possibly existing in biological samples. The method comprises the following steps of: amplifying nucleic acid segments of the biological samples; detecting by using a dye reagent; and observing a detection result by naked eyes or detecting a detection result by means of ultraviolet light. The invention also provides a primer used for amplification and a complete set of device for detection. In the method, the result is directly observed by the naked eyes or by utilizing the ultraviolet light; and the detector has the advantages of simple operation, wide sample range and convenient carrying, detection on the first site where the epidemics happen, and suitability for early diagnosis of various pathogens including respiratory infectious disease.