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43results about How to "Improve survival rate" patented technology

Antigen-specific T lymphocyte freezing medium and preparation method and application thereof

ActiveCN107148967AReduce operating proceduresLow pollution rateDead animal preservationSodium Chloride InjectionT lymphocyte
The invention provides an antigen-specific T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte electrolyte injection, 30-40% of glucose and sodium chloride injection, 5-15% of dextran glucose injection and 15-25% of human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte electrolyte injection, 20-30% of glucose and sodium chloride injection, 5-15% of dextran glucose injection, 15-25% of human albumin solution and 10-20% of dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the antigen-specific T lymphocyte freezing medium. The antigen-specific T lymphocyte freezing medium is capable of enabling less crystal to form in cells, increasing cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T lymphocyte freezing medium and antigen-specific T lymphocyte injection.
Owner:CHENZHOU BINZE MEDICAL LAB CO LTD

Sectional production method of Litsea coreana insect tea

ActiveCN112868856AConducive to drilling and feedingImprove survival rateAnimal feeding stuffTea substituesBiotechnologyCorn flour
The invention discloses a sectional production method of Litsea coreana insect tea, and belongs to the technical field of insect tea production. The method comprises the following steps: dispersing eggs of a tea-producing insect aglossa dimidiata in a low-age larva foodstuff for culturing; after 3-instar larvae are obtained, continuously culturing the larvae with advanced-age larva foodstuff; after the tea-producing insects begin to emerge, collecting larva excrement, and removing impurities to obtain the litsea coreana insect tea. The low-age larva foodstuff is obtained by taking young eagle tea shoots as raw materials, fermenting, drying and crushing to obtain eagle tea powder, and then mixing the eagle tea powder with corn flour and soybean flour; wherein the foodstuff for the elderly larvae is litsea coreana fragments; and the water content of the low-age larva foodstuff and the water content of the high-age larva foodstuff are both 20 wt%. According to the method, the insect tea is obtained by improving the foodstuff of the larvae of the tea-producing insects, strictly controlling the water content of the foodstuff and carrying out segmented culture on the larvae, so that the survival rate of the tea-producing insects is effectively improved, the tea sand particles of the obtained insect tea are uniform, and the quality is improved.
Owner:GUIZHOU INST OF BIOLOGY

Cuttage method of rose

InactiveCN103960006AEasy accessShorten rooting timeHorticultureBiologySurvival rate
The invention discloses a cuttage method of rose. The method includes the steps of first, selecting and making cuttings; second, preparing nutrient solution; third, making cutting blocks; fourth, performing cuttage, namely inserting made rose stems into flower mud, pouring the cuttage nutrient solution into a plastic tray, placing the rose stems, inserted into the flower mud, in the plastic tray, ensuring one third of each cutting block is immerged in the nutrient solution, placing the plastic tray in the environment with 2000-3000lux illumination and good ventilation, and moving the plastic tray with the flower mud into a flowerpot after all cuttings root. The method has the advantages that required materials are convenient to obtain, and no high technical demands are require; unlike all-light spraying requiring many automatic devices, the method requires regular addition of the nutrient solution only; rooting is fast; antibiotics are continuously used in the cuttage process, invasion of pathogens into cuttings can be controlled effectively, cutting quality is improved, and fewer cuttings degrade; transplanting survival rate is high.
Owner:HUOCHENG COUNTY KEFA TECH RISK INVESTMENT

Seedling-raising substrate for decreasing insect damage in snake gourd planting process and preparation method thereof

InactiveCN106134834AEnhance matrix looseningImprove survival rateBio-organic fraction processingBiological combination treatmentBacteriaBiology
The invention discloses a seedling-raising substrate for decreasing insect damage in the snake gourd planting process. The seedling-raising substrate is prepared from, by weight, 15-22 parts of snake gourd peels, 9-15 parts of attapulgite, 7-16 parts of kaolin, 1-3 parts of vermiculite mixture, 3-5 parts of humic acid, 6-12 parts of peat, 5-10 parts of corn straws, 5-8 parts of cassava straws, 4-7 parts of fermented vinegar dregs, 5-9 parts of crushed vinegar dregs, 7-12 parts of mushroom residues, 6-9 parts of wormcast, 14-23 parts of chicken manure, 11-18 parts of cow dung, 4-9 parts of calcium superphosphate, 12-15 parts of sulfate, 4-6 parts of VT-aerobic fermentation bacteria, 6-8 parts of potassium bacterium fungicide and 5-11 parts of EM fungicide. The raw materials for preparing the seedling-raising substrate are wide in source and low in cost, the attapulgite, the kaolin and the vermiculite mixture are preprocessed, the loose and transparent properties of the substrate are strengthened, seedling growth and development are promoted, and the survival rate of seedlings is improved. The snake gourd seedlings cultured by adopting the substrate are low in disease and insect pest occurrence rate and are healthier and stronger.
Owner:QIANSHAN YOUYU TRICHOSANTHES KIRILOWII MAXIM DEV CO LTD

Cultivation method for sugarcane seedlings

InactiveCN107950314AEasy to confirmImprove survival rateCulture mediaFruit crop cultivationGerminationGrowth speed
The invention discloses a cultivation method for sugarcane seedlings. The method comprises the following steps that a seedling bed capable of storing water and draining water is built; firstly, a layer of bottom mud is laid above the seedling bed, water is sprayed to the bottom mud, and then the seedling bed is put under the blazing sun for quite a long time; then, a layer of sludge is laid abovethe bottom mud, ditches are dug in the two sides of the seedling bed, and the sludge is dried in air; finally, a layer of composite soil is laid above the sludge; seeds are sown above the composite soil; then, a plastic small shed is set up above the seedling bed; then, seedling management is performed, and after sugarcane seedlings grow to 15-20 cm, provisional planting and field planting are performed. According to the cultivation method for sugarcane seedlings, the operation method is simple, equipment and raw materials do not need to be specially machined, few manpower and material resources are consumed, the cost is low, the obtained sugarcane seedling germination rate is high, the growth speed is high, the sugarcane seedlings are robust, the root system is developed, the number of leaves is large, the transplanting survival rate is high, and the method has the good economic, social and ecological benefits and is suitable for large-scale application and popularization.
Owner:SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI

Cutting seedling raising method of cherry trees

InactiveCN106888915AHigh reproductive coefficientImprove survival rateCultivating equipmentsHorticulture methodsAgroforestryCherry tree
The invention relates to a cutting seedling raising method of cherry trees. The method comprises the steps that a young and robust mother tree is selected, annular primarily-lignified branches are cut into cuttings with the length of 10-15 cm, the lower ends of the cuttings are sliced, oblique notches are scratched at the portions 0.7-1 cm away from the lower ends of the cuttings, and the depth of the notches is 1-2 mm; the lower portions of the cuttings are inserted into an ABT number 6 solution with the concentration of 200 mg/L to be soaked; after the cuttings are aired at a shade place, the lower ends of the cuttings are cut off and inserted into a seedbed, and when cuttage is conducted, punching is conducted with a wood drill a little thicker than the cuttings, and then the cuttings are put into holes to be compacted; the seedbed is erected over the ground, and after cottage is completed, the seedbed is smoked by laying smoking materials on the ground and then thoroughly watered; the temperature of the seedbed is kept at 20 DEG C to 25 DEG C, and after 3-4 weeks, transplanting can be conducted when 3-5 roots grow on each cutting. According to the cutting processing method and the smoking method, the survival rate of cutting seedling raising of the cherry trees can be effectively increased, and the disease-infected seedlings can be effectively reduced.
Owner:湖州尚翔生态农业股份有限公司

Planting method of torreya grandis

InactiveCN105613184AEasy to operateImprove survival rateCultivating equipmentsGraftingBiologyDiurnal temperature variation
The invention provides a planting method of torreya grandis. The method comprises four steps of accelerating germination, grow seedlings, transplanting and grafting, wherein in the accelerating germination stage, a two-layered greenhouse is used; an inner-layer plastic film of the two-layered greenhouse is a two-layered film; a plurality of air cavities are formed in the middle of the two-layered film; the two-layered film is aerated or deflated according to the temperature difference between day and night, so that the temperature in the greenhouse is adjusted. A pit 20 cm in depth is excavated in the greenhouse; a first isolation net layer is paved at the bottom of the pit; torreya grandis seeds are piled on the first isolation net layer; a second isolation net layer is paved on the torreya grandis seed layer; a sandy soil layer is paved on the second isolation net layer. By the planting method of torreya grandis provided by the invention, manual temperature control is performed for half a month in December, the sprouting rate in the next January can be 60 percent, and can be 80 percent in next February; two sunshade nets are placed in the pit, so that ventilation is ensured, and the covering soil layer and the torreya grandis seed layer can be taken away conveniently; the process is simple; time and labor are saved.
Owner:安徽省丰谷香榧有限公司

Ligusticum wallichii cultivation method capable of reducing plant pest and disease damage and increasing survival rate and yield

InactiveCN104705066AHarm reductionImprove survival rateCultivating equipmentsPlant protectionDisease damageLigusticum
The invention discloses a ligusticum wallichii cultivation method capable of reducing plant pest and disease damage and increasing survival rate and yield. The method comprises the following steps: sequentially performing rice seedling planting and rice harvesting, regular soil preparation, poria seed planting, field management and ligusticum wallichii harvesting within a planting period; specifically, in the regular soil preparation, performing immersed soaking treatment on farmland with rice harvested, soaking and draining accumulated water out of the field, ploughing, then finely raking, flattening surface soil, ditching and excavating shallow trenches; in the poria seed planting step, performing cuttage on poria seeds at the bottom of the shallow trenches, and immediately covering with straws along the shallow trenches after cuttage; and in the field management step, supplementing seedlings, weeding, draining water, applying fertilizer and performing pest and disease prevention. According to the ligusticum wallichii cultivation method, the block stem rotten sickness and grub damage of the ligusticum wallichii can be effectively reduced, the physiological seedling death and withering are effectively prevented, the purpose of returning straws to the field can be achieved, the root development of the ligusticum wallichii can be promoted, the field weeds can be reduced, and the yield of the ligusticum wallichi can be effectively increased.
Owner:SICHUAN NEO GREEN PHARMA TECH DEV

Additive used for increasing clone number of hybridoma cells and preparation method of additive

ActiveCN105462934APromote growthGrow fastFused cellsCell culture active agentsUltrafiltrationHybridoma cell
The invention provides an additive used for increasing the clone number of hybridoma cells and a preparation method of the additive, and relates to an immunological research technology. The preparation method includes the following steps that 1, culture solution supernatant for eukaryotic cells capable of expressing IL-6 protein is acquired, wherein the cell culture solution supernatant contains expressed IL-6 protein; 2, the cell culture solution supernatant is subjected to ultrafiltration and filtration sterilization in sequence, and a cell culture solution supernatant concentrated solution is obtained; 3, sodium selenite is added into the cell culture solution supernatant concentrated solution, and the additive is obtained. As proved by experimental data, the clone number of the hybridoma cells can be increased by nearly 5 times after the additive is adopted. The invention further provides a hybridoma cell culture medium containing the additive.
Owner:TARCINE BIOMED INC

Tomato seedling cultivation method for obviously increasing yield at early period

ActiveCN109287461AIncrease productionImprove survival rateGraftingSaving energy measuresAgricultural scienceSeedling
The invention belongs to the technical field of agriculture and particularly relates to a tomato seedling grafting method for obviously increasing the yield at the early period and a matched cultivation measure. A tomato seedling after grafting has two fruiting scions, and seedling raising and cultivation, light supplement and healing and water and fertilizer management are conducted. According tothe method, the tomato yield (first two scion fruits) at the early period is obviously higher than the yield of a traditional grafting method, and compared with a traditional management method, the flowering phase is advanced by 4-15 days.
Owner:FUJIAN AGRI & FORESTRY UNIV

Intelligent lifesaving floating plate

ActiveCN112896465AIncrease chances of survivalSafe waitingBatteries circuit arrangementsLife-buoysMarine engineeringElectric machine
The invention discloses an intelligent lifesaving floating plate which comprises a floating plate body, three heat conduction strips are fixedly connected to the top of the floating plate body, and solar power generation pieces are fixedly installed at the positions, located on the front sides and the rear sides of the three heat conduction strips, of the top of the floating plate body. The invention relates to the technical field of on-water lifesaving equipment. According to the intelligent lifesaving floating plate, through solar power generation, endurance heating of the heat conduction strip and maintenance of the basic survival body temperature of a survival person, an alarm mechanism is directly triggered, a distress signal is diffused to the outside, and in cooperation with the arrangement of a motor, a take-up wheel, a power transmission pull rope, a backpack air bag and a control button, after the person grasps the backpack air bag or the power transmission pull rope, by pressing the control button, the floating plate body is moved to the position where personnel are located, so that the personnel in water can quickly find the lifesaving equipment, the personnel can safely wait for the arrival of rescue personnel on the sea, and the survival probability of the personnel falling into water is greatly improved.
Owner:ZHEJIANG KRESS IND & TRADE CO LTD

Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling

ActiveCN105638462AEasy to transplantImprove survival rateCultivating equipmentsCulture mediaChaffEnvironmental temperature
The invention discloses a method for domesticating and transplanting a taxus chinensis var mairei tissue culture seedling. The method comprises the following steps of (1) selecting the tissue culture seedling by means of selecting the taxus chinensis var mairei tissue culture seedling with a well-grown root system and more than one true leaf; (2) preliminarily domesticating the tissue culture seedling by means of putting a tissue culture container with the tissue culture seedling into a greenhouse for 3 to 5 days; (3) preparing a transplanting container and a culture substrate by means of selecting a seedling culturing box, and filling vermiculite, turf or nonwoven-fabric wrapped fresh coconut chaff in holes of the seeding culturing box; (4) transplanting and hardening seedling by means of planting the tissue culture seedling in the middle of the culture substrate obtained in the step (3), watering sufficiently, closing a seedling culturing box cover, putting the box into the greenhouse with 50 percent of sunshield screen, culturing at an environmental temperature of not more than 35 DEG C, and opening the seedling culturing box cover and performing a conventional seedling management mode after two weeks, wherein the survival rate of transplanted tissue culture seedlings reaches 100 percent at most. The method disclosed by the invention has the advantages that the disadvantages in the prior art are overcome, and the effects of simplicity in operation, low cost, high efficiency, stability and reusability of the seedling culturing box are achieved.
Owner:HUNAN AGRICULTURAL UNIV

Production method of pyramidalis gardening quadripod

The invention discloses a manufacture method of pyramidalis gardening quadripod, and belongs to the technical field of farm garden. The method comprises the steps of preparing a seedling bed, preparing shoot for cuttings, cutting, carrying out post-cutting management, selecting seedlings, planting for the first time, planting for the second time, planting for the third time, carrying out planting cultivation, designing a process size of the gardening quadripod, selecting a tree according to the quadripod, transplanting the tree, carrying out the post-planting management, facilitating growth of the tree, binding the tree by utilizing iron wires and a reinforcement net according to a designed pattern of each part, shaping and reinforcing four sides of the quadripod body, and carrying out the growth maintenance to obtain the pyramidalis gardening quadripod. By adopting the production method, the survival rate of the seedling is high, and the produced gardening quadripod is good in shape, is not deformed in the transplanting process, easy to maintain after the tree is transplanted and capable of facilitating the long-term growth of the tree.
Owner:丁洁

Potato plantation method

InactiveCN106550748AHigh porosityConducive to cultivationPlant cultivationCultivating equipmentsComing outInsect pest
The invention discloses a potato plantation method. The potato plantation method comprises following steps of A, carrying out soil preparation, applying decomposed farmyard manure, calcium superphosphate, plant ash and organic trace elements after a deep ploughing process; carrying out fine ploughing and leveling processes; B, carrying out a sowing process, cutting potatoes into cubes, wherein each potato cube at least reserves a middle bud or terminal bud, and the terminal bud is longitudinally cut and equally distributed to 2-4 potato cubes; putting the cut potato cubes on a wet, shady, cool and ventilated place timely for airing, immersing the potato cubes in a sprouting agent after wound healing, and then carrying out the sowing process; C, carrying a topdressing process according to the weight ratio of nitrogen, phosphor and potassium being 5:2:1 in dependence on the soil fertility condition; D, carrying out field management, carrying out shallow irrigation once after potato seedlings come out evenly, carrying out final irrigation once 60-70 days after seedling emergence, carrying out irrigation in dependence on rainfall and soil humidity conditions afterwards, removing weeds when we see at any moment, and controlling plant diseases and insect pests timely; and E, carrying out harvest timely after potatoes are ripe. By employing the potato plantation method, the output can be increased, the potatoes are green and pollution-free, and the potato plantation method is simple and easy to operate.
Owner:覃忠强

Cattle feed additive

InactiveCN107307197AImprove meat qualityImprove disease resistanceFodderYeastCattle feeding
The invention discloses a cattle feed additive. The cattle feed additive consists of the following raw materials in parts by weight: 4-16 parts of perilla frutescens, 2-20 parts of cyrtomium rhizomes, 10-30 parts of cod-liver oil, 2-10 parts of sodium selenite, 3-13 parts of Haixiao, 5-25 parts of radix polygalae, 2-12 parts of longan aril, 4-20 parts of Chinese yam rhizomes, 5-25 parts of vietnamese sophora roots, 3-17 parts of folium isatidis, 2-12 parts of endothelium corneum gigeriae galli, 4-16 parts of actinolitum brevifibrum, 2-12 parts of herba asari, 4-12 parts of origanum vulgare, 3-13 parts of Chinese pulsatilla roots, 4-16 parts of fleeceflower roots, 2-18 parts of radix achyranthis bidentatae, 1-9 parts of a bacillus natto agent, 2-10 parts of bacillus and 1-7 parts of active dry yeast. The cattle feed additive disclosed by the invention has the advantages of being rich in nutrition, capable of improving beef quality, increasing the survival rate of cattle and the like.
Owner:无为县松林生态养殖有限公司

Biological detention pond for improving ability of nitrogen and phosphorus removal

InactiveCN109809632AEfficient removalIncrease seepage pathWater contaminantsMultistage water/sewage treatmentNitrogen removalNitrogen
The invention provides a biological detention pond for improving the ability of nitrogen and phosphorus removal. The biological detention pond includes a water reserve and a detention pond body structure arranged on the lower part of the water reserve, the detention pond body structure includes a planting soil layer, a packing area and a pebble bed from top to bottom, wherein the packing area is internally provided with a plurality of layers of impermeable geotechnical cloths, the detention pond body structure is divided into a plurality of layers of corridors, one ends of the impermeable geotechnical cloths are connected to the inner wall of the detention pond body structure, the other ends of the impermeable geotechnical cloths are free ends, a serpentine water seepage passageway is formed among the multiple layers of corridors, and the multiple layers of corridors are filled with different fillers correspondingly; and the pebble bed communicates with the water seepage passageway, and a perforated drainage pipe is arranged in the pebble bed. According to the biological detention pond for improving the ability of nitrogen and phosphorus removal, through design of the multiple layers of corridors, the hydraulic retention time of rainwater in the detention pond can be increased, phosphorus removal is facilitated, entering of oxygen can be isolated, an anoxic zone and an anaerobic zone are arranged on the lower part of the biological detention pond, and nitrogen removal is facilitated.
Owner:JIANGSU UNIV

High-yield cultivation technique for black agaric

InactiveCN105557296ATender meatImprove survival rateHorticultureWeedTerrain
The invention provides a high-yield cultivation technique for black agaric, belonging to the field of agriculture, and specifically relates to a cultivation method for the black agaric. The invention provides the high-yield cultivation technique for the black agaric with high quality and high yield. The high-yield cultivation technique for the black agaric comprises the following steps: (1) selection of excellent strains, wherein the meaning of the excellent strains comprises two aspects, i.e., first, inherent characteristics of strains are excellent, and second, the quality of the strains is good; (2) field selection, wherein a field is selected in a leeward and sunny place near agaric forest, and the field requires shallow weeds, flat terrain and long sunshine; (3) inoculation, wherein inoculation is a key work of survival of black agaric and directly influences the problem of future yield of the black agaric, so the step of inoculation is significantly important; (4) field-planting management, a step of carrying out spawn running by stacking up, and subjecting strain-inoculated agaric sticks to pigsty stacking or triangular stacking; and (5) stack scattering and field arraying, a step of arraying agaric sticks on the ground after inoculation, wherein a distance between every stick is about 1 inch, but the sticks need to be turned over every ten days.
Owner:申茂军

Rapid propagation method of taxus mairei

The invention relates to a plant propagation method, in particular to a rapid propagation method of taxus mairei. The method comprises the following steps of selecting an explant; sterilizing the explant; inducing buds, wherein a bud induction culture medium is an MS culture medium in which 0.3-0.5 mg / L of NAA, 1.3-1.7 mg / L of 6-BA, 1g / L of activated carbon, 30 g / L of sucrose and 7 g / L of agar areadded; performing root induction, wherein a rooting culture medium is a 1 / 2 MS culture medium in which 0.4-0.6 mg / L of KT, 1.8-2.2 mg / L of NAA, 90-110 MG / l of AgNO3, 1 g / L of activated carbon, 7 g / Lof agar and 30 g / L of sucrose are added; domesticating tissue culture seedlings. The method improves the survival rate and rooting rate of tissue culture, shortens the propagation time and improves the propagation efficiency.
Owner:JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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