Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling

A technology of southern yew and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, cultivation, etc., to achieve the effects of large-scale production, short hardening time, and convenient transplanting

Active Publication Date: 2016-06-08
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on a quick and easy domestication and transplanting method for in vitro germination of Taxus chinensis tissue seedlings

Method used

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  • Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling
  • Method for domesticating and transplanting taxus chinensis var mairei tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Preferred Embodiment 1

[0033] (1) Selection of tissue culture seedlings: select Taxus chinensis tissue culture seedlings with well-developed root systems and more than one true leaf;

[0034] (2) Preliminary domestication of tissue culture seedlings: place the tissue culture container with tissue culture seedlings in the greenhouse for 3-5 days;

[0035] (3) preparation of transplanting container and cultivation substrate: select seedling box, and fill vermiculite in the hole of seedling box;

[0036] (4) transplanting and hardening: the tissue culture seedlings are implanted in the middle of the cultivation medium in step (3), water is poured enough, the cover of the seedling raising box is closed, put into a greenhouse with 50% sunshade net for cultivation, and the ambient temperature The temperature does not exceed 35°C. After 2 weeks, the lid of the seedling box can be opened to enter the conventional seedling production management mode. The survival rate of tissue

Example Embodiment

[0037] Preferred Embodiment 2

[0038] (1) Selection of tissue culture seedlings: select Taxus chinensis tissue culture seedlings with well-developed root systems and more than one true leaf;

[0039] (2) Preliminary domestication of tissue culture seedlings: place the tissue culture container with tissue culture seedlings in the greenhouse for 3-5 days;

[0040] (3) preparation of transplanting container and cultivation substrate: select seedling box, fill peat in the hole of seedling box;

[0041] (4) transplanting and hardening: the tissue culture seedlings are implanted in the middle of the cultivation medium in step (3), water is poured enough, the cover of the seedling raising box is closed, put into a greenhouse with 50% sunshade net for cultivation, and the ambient temperature The temperature does not exceed 35°C, and the lid of the seedling box can be opened after 2 weeks to enter the conventional seedling production management mode. The survival rate of tissue culture

Example Embodiment

[0042] Preferred Embodiment 3

[0043] (1) Selection of tissue culture seedlings: select Taxus chinensis tissue culture seedlings with well-developed root systems and more than one true leaf;

[0044] (2) Preliminary domestication of tissue culture seedlings: place the tissue culture container with tissue culture seedlings in the greenhouse for 3-5 days;

[0045](3) preparation of transplanting container and cultivation substrate: select seedling box, fill non-woven wrapping fresh coconut bran powder in the hole of seedling box;

[0046] (4) transplanting and hardening: the tissue culture seedlings are implanted in the middle of the cultivation matrix in step (3), water is poured enough, the seedling box cover is closed, and the seedlings are put into a greenhouse with 50% sunshade net for cultivation. The temperature does not exceed 35°C, and the lid of the seedling box can be opened after 2 weeks to enter the conventional seedling production management mode. The survival rate

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Abstract

The invention discloses a method for domesticating and transplanting a taxus chinensis var mairei tissue culture seedling. The method comprises the following steps of (1) selecting the tissue culture seedling by means of selecting the taxus chinensis var mairei tissue culture seedling with a well-grown root system and more than one true leaf; (2) preliminarily domesticating the tissue culture seedling by means of putting a tissue culture container with the tissue culture seedling into a greenhouse for 3 to 5 days; (3) preparing a transplanting container and a culture substrate by means of selecting a seedling culturing box, and filling vermiculite, turf or nonwoven-fabric wrapped fresh coconut chaff in holes of the seeding culturing box; (4) transplanting and hardening seedling by means of planting the tissue culture seedling in the middle of the culture substrate obtained in the step (3), watering sufficiently, closing a seedling culturing box cover, putting the box into the greenhouse with 50 percent of sunshield screen, culturing at an environmental temperature of not more than 35 DEG C, and opening the seedling culturing box cover and performing a conventional seedling management mode after two weeks, wherein the survival rate of transplanted tissue culture seedlings reaches 100 percent at most. The method disclosed by the invention has the advantages that the disadvantages in the prior art are overcome, and the effects of simplicity in operation, low cost, high efficiency, stability and reusability of the seedling culturing box are achieved.

Description

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Claims

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Application Information

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Owner HUNAN AGRICULTURAL UNIV
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