Rapid propagation method of taxus mairei

[0016] A kind of rapid propagation method of southern yew, comprising the following steps:

[0017] (1) Select the 5-10-year-old southern yew plant as the mother plant, and cut it from the mother plant as an explant before the swelling scales of the buds of the year have not cracked;

[0018] (2) Disinfect the clipped explants with 75% alcohol for 30s on the ultra-clean workbench, pour off the alcohol, rinse with sterile water for 3-4 times, and then rinse with 0.1% HgCl 2 Disinfect for 5-15 minutes, and finally rinse with sterile water;

[0019] (3) Inoculate the washed explants on the bud induction medium and cultivate them for 15 days, the culture temperature is 24°C, the daily light time is 12 h, and the light intensity is 1800-2000 lx; ​​the bud induction medium is MS+ 0.4 mg / L NAA + 1.5 mg / L 6-BA + 1g / L activated carbon + 30 g / L sucrose + 7g / L agar;

[0020] (4) Take out the new shoots when they grow to 2-3cm, soak the base of the new shoots with a concentration of 50...

[0023] A kind of rapid propagation method of southern yew, comprising the following steps:

[0024] (1) Select the 5-10-year-old southern yew plant as the mother plant, and cut it from the mother plant as an explant before the swelling scales of the buds of the year have not cracked;

[0025] (2) Disinfect the clipped explants with 75% alcohol for 30s on the ultra-clean workbench, pour off the alcohol, rinse with sterile water for 3-4 times, and then rinse with 0.1% HgCl 2 Disinfect for 5-15 minutes, and finally rinse with sterile water;

[0026] (3) Inoculate the washed explants on the bud induction medium and cultivate them for 17 days at a culture temperature of 25°C, with a daily light time of 14 h and a light intensity of 1800-2000 lx; ​​the bud induction medium is MS+ 0.3 mg / L NAA + 1.3 mg / L 6-BA + 1g / L activated carbon + 30 g / L sucrose + 7g / L agar;

[0027] (4) Take out the new shoots when they grow to 2-3 cm, soak the base of the new shoots with a concentration of 5...

[0030] A kind of rapid propagation method of southern yew, comprising the following steps:

[0031] (1) Select the 5-10-year-old southern yew plant as the mother plant, and cut it from the mother plant as an explant before the swelling scales of the buds of the year have not cracked;

[0032] (2) Disinfect the clipped explants with 75% alcohol for 30s on the ultra-clean workbench, pour off the alcohol, rinse with sterile water for 3-4 times, and then rinse with 0.1% HgCl 2 Disinfect for 5-15 minutes, and finally rinse with sterile water;

[0033] (3) Inoculate the rinsed explants on the bud induction medium and cultivate them for 20 days, the culture temperature is 26°C, the daily light time is 15 h, and the light intensity is 1800-2000 lx; ​​the bud induction medium is MS+ 0.5 mg / L NAA + 1.7 mg / L 6-BA + 1g / L activated carbon + 30 g / L sucrose + 7g / L agar;

[0034] (4) Take out the new shoots when they grow to 2-3 cm, soak the base of the new shoots with 500 mg / L IBA solutio...