The invention provides an
antigen-specific
T lymphocyte freezing medium, comprising freezing medium A and freezing medium B; the freezing medium A includes, by volume, 30-40% of plasmalyte
electrolyte injection, 30-40% of glucose and
sodium chloride injection, 5-15% of
dextran glucose injection and 15-25% of
human albumin solution; the freezing medium B includes, by volume, 20-30% of plasmalyte
electrolyte injection, 20-30% of glucose and
sodium chloride injection, 5-15% of
dextran glucose injection, 15-25% of
human albumin solution and 10-20% of
dimethyl sulfoxide; the freezing medium A and the freezing medium B are stored separately; in use, the freezing medium A and the freezing medium B are mixed in a ratio of 1:(0.5-2), forming the
antigen-specific
T lymphocyte freezing medium. The
antigen-specific
T lymphocyte freezing medium is capable of enabling less
crystal to form in cells, increasing
cell survival rate and maintaining tumor-killing ability of cells. The invention also provides a preparation method and application of the antigen-specific T
lymphocyte freezing medium and antigen-specific T
lymphocyte injection.