12 results about "Inoculation" patented technology

The invention discloses a ginsenoside-containing vegetable oil adjuvant and a preparation method and application thereof. The ginsenoside-containing vegetable oil adjuvant is characterized in that edible vegetable oil is used as the adjuvant oil; the ginsenoside-containing vegetable oil adjuvant comprises 10 to 100mu g/mL ginsenoside. According to the preparation method, the ginsenoside-containing vegetable oil is utilized for preparing vaccines, so that the vaccine safety can be greatly improved, and the potential hazard of the general mineral oil adjuvant on food hygiene is avoided; after being mixed, ginsenoside and vegetable oil play the effect of collaborating with the adjuvant, thus the valence of antibody is raised by 4.7 times, and the antibody level of the vaccines is also greatly improved; in addition, the prepared vaccine is high in mobility, convenient for inoculation, small in local stimulation, and thus relieving pain on animals; when the ginsenoside-containing vegetable oil adjuvant is applied to vaccine production, the existing production process is remained, and the method is simple and convenient.

The invention aims to provide a microfluidic chip system for culture and multiplication behavior research of marine microalgae. Inoculation, batch culture, semi-continuous chemostat culture of the marine microalgae and a marine microalgae population multiplication behavior online monitoring process are integrated on a functional chip to be completed by means of fabrication of a scale integration chip and flexible combination of multiple cell technologies on the chip. The microfluidic chip system consists of two elementary units, wherein the first elementary unit refers to the microfluidic chip for culture and multiplication behavior research of the marine microalgae, and the second elementary unit refers to microfluidic chip peripheral equipment for culture and multiplication behavior research of the marine microalgae. The microfluidic chip system has the advantages that the microfluidic chip system is high in throughput, simple and convenient to operate and low in cost and is a novel platform for marine microalgae culture, population multiplication research and correlational research fields of using microalgae as experimental subjects.

The invention discloses a culture method and a culture container for an in-vitro sterile host plant-mycorrhizal edible fungus intergrowth seedling. The culture method comprises the steps of (1) preparing an in-vitro sterile host plant; (2) sterilely and accurately inoculating; and (3) culturing the host plant-mycorrhizal edible fungus intergrowth seedling. The culture container comprises a transparent container, a container cover and a triangular fixing frame. A filtration ventilating window is arranged on the container cover; the triangular fixing frame is arranged on the bottom part of the container; a circular ring with a notch is arranged on the upper part of the triangular fixing frame; and a plurality of outwards hooks for fixing an elastic rubber ring are arranged on the upper part of the circular ring. According to the culture method and the culture container for the in-vitro sterile host plant-mycorrhizal edible fungus intergrowth seedling provided by the invention, a host plant system can be cultured under the controlled environment conditions of temperature, humidity and illumination, so that the intergrowth seedling can be bred quickly and largely; and compared with the prior art, the breeding speed is improved by 3 to 12 times, the inoculation success rate is improved by 1.6 to 2.5 times, and the production rate of the inoculated host plant-mycorrhizal edible fungus intergrowth seedling is improved by 30 percent to 50 percent.

The invention provides a wheat scab resistance identification method. Wheat scab resistance is identified in a short time at high throughput in case of controllable environmental conditions by utilizing in-vitro ears of wheat. The method disclosed by the invention has the advantages that a novel scab resistance evaluation standard is discovered, and the wheat scab resistance is evaluated accordingto whether black dense necrotic spots are produced at inoculation positions. By utilizing the standard, limitations of external environmental conditions can be avoided, and the wheat scab resistancecan be simply and rapidly identified within 4-5 days. The method is intuitive and reliable in phenotype identification and excellent in repeatability, is capable of completing resistance identification of batches of materials in a short time, and has significances on genetics and breeding researches of wheat scab resistance all over the world.

The invention relates to a one-time seedling growing method for polygonatum sibiricum. The method includes the first step of culture medium preparation, the second step of explant selection and sterilization, the third step of explant inoculation, the fourth step of culture, and the fifth step of transplanting and hardening. The one-time seedling growing method for polygonatum sibiricum is convenient and fast to implement. Compared with a traditional method that underground rhizomes are selected for tissue culture, tender leaves of polygonatum sibiricum are selected as explants for tissue culture, materials are easy to obtain, and the economic benefit brought by rhizomes of polygonatum sibiricum is not influenced; besides, as the number of leaves of polygonatum sibiricum is large and the propagation coefficient is higher, the production method is convenient and fast to implement, and tissue culture seedlings of polygonatum sibiricum can be cultured within a short time. In addition, the method is easy and convenient to implement, only one step is needed from explants to seedlings, manual work is greatly reduced, and contamination is reduced to the maximum; moreover, by selecting a fine female parent for germplasm purification and rejuvenation of polygonatum sibiricum and maintenance of superior characters, the method has high application value on propagation of fine seedlings of polygonatum sibiricum.

The present invention provides a management system for utilizing code identify management insurance inoculation product. The system includes a system computer, a terminal, a printer, an input device, a product code information database, a code generator, a product code table database, a product code scutcheon, a product warehouse management system, a product financial management system, a product object picture database, a manufacturer information database, a management of vaccinate site, a safety monitoring system of vaccinate. Since using the product code scutcheon provided by the invention as identification card preventing the inoculation goods access the management system, the register product code replaces the previous sorts, factory, preparation formulation, norm, technics etc of the register goods, the working efficiency is enchanced 4 times and reducing the error, and achieving the data share of each business module of the vaccinate administration section, having important meanings to improve medical management level and the disease prevention control Strategy research.

An abalone mushroom cultivation technology comprises the steps of 1, mechanical mixing and bagging; 2, separate and centralized sterilization; 3, three-level purification and inoculation; 4, intelligent mushroom house cultivation; 5, abalone mushroom house fruiting stage fertility management. The optimal formula and fruiting management control mode suitable for abalone mushroom cultivation are adopted, a foundation is laid for stable and high yield, the biological efficiency of finished mushroom is 55% or above, mushroom is round and has a good form, selling price is effectively increased by 20% or above, sustained and efficient market supply is ensured, and comprehensive benefits are increased by 25% or above.

The application of the invention discloses a golden cleistothecium shaped ascospore reproducing technology of bulk tea having golden cleistothecium shaped ascospores. The golden cleistothecium shapedascospore reproducing technology comprises the following steps of rehydrating raw materials, performing sterilization, performing cooling, performing inoculation, performing golden cleistothecium shaped ascospore reproducing and performing drying, wherein in the sterilization step, high-temperature high-pressure sterilization treatment is adopted, the sterilization temperature is 110-120 DEG C andthe sterilization time is 15-30min. According to the golden cleistothecium shaped ascospore reproducing technology of bulk tea having golden cleistothecium shaped ascospores provided by the application, prepared tea leaves are sweet in mouth feel, aroma types of fermented young leaves are rich, the aroma of the fermented young leaves contain flower aroma and sweet aroma, bacterium flower aroma isrich, and the golden cleistothecium shaped ascospore reproducing technology is especially suitable for golden cleistothecium shaped ascospore reproducing of young leaves.

The invention discloses a full-automatic stream inoculation machine. The machine comprises a hopper, a level sensor, a motor driving module, a coupling, a screw feeding rod, a photoelectric sensor, a pressure sensor, a discharging pipe and a control cabinet, wherein the hopper is located above the level sensor; the motor driving module is located below the coupling; the coupling is connected with the screw feeding rod; the screw feeding rod is located above the photoelectric sensor; the photoelectric sensor is located above the discharging pipe; the pressure sensor is located on the inner side of the discharging pipe; the discharging pipe is located below the pressure sensor. The full-automatic stream inoculation machine communicates with data of a program of a pouring machine, when the pouring machine begins to pour molten iron, the full-automatic stream inoculation machine can control an inoculant to hit a molten iron flow through an inoculation material pipe, the inoculant enters a mold after full inoculation, and automatic and accurate inoculation is realized.

The invention discloses a simple and convenient identification method of bacillus cereus for konjac glucomannan, which comprises the following steps: step 1, sample preparation: weighing a konjac glucomannan raw material sample under a sterile condition, adding normal saline, and stirring for dissolving; step 2, pre-screening targeted bacteria: putting the solution prepared in the step 1 into a constant-temperature furnace for heating and pre-cooking, and after the temperature is raised to 88-92 DEG C, carrying out heat preservation and pre-screening to obtain spore bacteria; step 3, sterile inoculation: sucking the solution pretreated in the step 2 into a sterile culture dish, adding a compound culture medium solution, and rotating the culture dish to uniformly mix; step 4, culturing: after the agar is solidified, turning over the plates, and culturing at 36 +/-1 DEG C for 48 +/-2 hours; and step 5, identifying, wherein cultured test colonies show colonies in the shape of snowflake or plum blossom. By means of the method, the raw materials containing the bacillus cereus and possibly having risks can be screened out in time within the shortest time, and the safety of jelly food is improved.