Endangered plant sphaeropteris lepifera propagation technology and field regression method

[0061] Example 1

[0062] One kind of tree fern propagation techniques includes the following steps:

[0063] (1) disinfection: fresh spores as the propagation material, the collected mature spores sterile water immersion 24h, filtered, and the spores into 1.5ml microcentrifuge tube, was added 1ml sterile water, placed in a centrifuge, 2500r / min rpm for 3min, the supernatant was removed again added dropwise 15% sodium hypochlorite and 10% hydrogen peroxide solution in the centrifuge tube by 3: 1 volume ratio mixed solution of 1ml, 4min stand sterilization, and rinsing with sterile water tree fern spores three times to give a sterile tree fern spore suspension;

[0064] (2) Pak inoculation: Turn the spore suspension after disinfecting to the germination medium surface, the germination medium: lower degreasing cotton, the intermediate 1 / 4 ms liquid medium, upper layer containing filter paper covering the entire petri dish Moisturize;

[0065] (3) spore growth: The dish is placed

[0068] Example 2

[0069] One kind of tree fern transplanting technique, wherein transplanting medium: peat, perlite and vermiculite, a volume ratio of 3: 1: 1, the organic matter content of 275.60g / kg, available phosphorus content of 0.052g / kg, K an amount of 0.23g / kg, available nitrogen content of 0.35g / kg, bulk density 0.23g / cm 3 The maximum water holding capacity was 373.7%;

[0070] After transplanting management measures: growth of 75% shade of 2-3 months, accept the normal growth of natural light.

[0071] Example 3

[0072] One kind of tree fern transplanting regression techniques and field planting method, wherein transplanting medium: peat, perlite and vermiculite, a volume ratio of 3: 1: 1, the organic matter content of 275.60g / kg, available phosphorus content of 0.052g / kg, of 0.23 g of potassium content / kg, of 0.35 g of available nitrogen / kg, bulk density 0.23g / cm 3 The maximum water holding capacity was 373.7%;

[0073] After transplanting management measures: 75% shading of growth 2-3 months after receiving the normal growth of natural light, and endangered plants be used in conjunction with the present invention, the cultivation temperature during guard shade.

[0074] When the tree fern grow to 1 - When 2 years old, to achieve the return field cultivation, temperature and humidity is provided an optical monitor, regular monitoring of the recording environment and seedling growth and development of the field return in summer high temperature exceeding