Method for building candidate marker diagnosis model for OA (osteoarthritis)

A diagnostic model and method establishment technology, applied in the fields of gene transcriptomics and proteomics, can solve the problems of inability to display deep cartilage and subchondral bone, and clinical application limitations

Inactive Publication Date: 2018-02-23
NANNING KECHENGHUI INFORMATION TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its clinical application is also limited due to the invasiveness of the examination and the inability to show changes in the deep cartilage and subchondral bone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] The establishment method of this OA candidate marker diagnostic model,

[0027] Consists of the following steps:

[0028] ①STR / ort model mouse serum; control mouse serum

[0029] ② iTRAQ labeling combined with MALDI-TOF MS / MS to discover and identify differential expression in serum;

[0030] ③Bioinformatics analysis and screening of candidate marker proteins;

[0031] ④Validation of OA candidate marker proteins:

[0032] Validation in serum of STR / ort model mice;

[0033] Validation in serum of OA patients;

[0034] Validation in the synovial fluid of OA patients;

[0035] ⑤ Detection of candidate markers in the serum of OA patients, and analysis of their relationship with the course of OA;

[0036] ⑥Establish the method of establishing the diagnostic model of OA candidate markers, and judge its significance in OA diagnosis and early warning of healthy people.

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Abstract

The invention discloses a method for building a candidate marker diagnosis model for OA (osteoarthritis). The method comprises the following steps: (1) building an STR / ort model mice serum, and contrasting mice serums; (2) finding and discovering serum difference expression through combination of iTRAQ marks and MALDI-TOF MS / MS; (3) analyzing and screening candidate marker protein through bioinformatics; (4) verifying OA candidate marker protein; (5) detecting candidate markers in serums of patients and analyzing relation between the candidate markers and OA disease courses; (6) establishing amethod for building the candidate marker diagnosis model for OA. According to the method disclosed by the invention, markers are confirmed through samples of population, values for diagnosing OA by the OA candidate markers are evaluated and correlation between the candidate marker diagnosis model and clinics is analyzed, and the feasibility of the candidate marker diagnosis model on clinical application such as prevention and early diagnosis to OA and a meaning of the candidate marker diagnosis model in health alarm of OA of the population are proposed.

Description

technical field [0001] The invention relates to the fields of gene transcriptomics and proteomics, in particular to a method for establishing a diagnostic model of OA candidate markers. Background technique [0002] Osteoarthritis (OA), also known as degenerative bone joint disease, is one of the most common joint diseases, mainly manifested as slowly developing pain, stiffness, swelling of the joints, accompanied by joint dysfunction, and even disability[1- 2]. The pathogenesis of OA is that joints are affected by genetic, biological and mechanical events (such as genetics, metabolic abnormalities, strain, trauma, etc.), which induce changes in cells and matrix in terms of morphology, biomechanics, and molecules. It leads to an imbalance in the degradation and synthesis of articular chondrocytes, extracellular matrix and subchondral bone. The outcome is softening, fibrosis, ulceration and loss of chondrocytes. After losing the protective effect of cartilage, the subchondr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
Inventor 于思创王海云
Owner NANNING KECHENGHUI INFORMATION TECH CO LTD
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