11 results about "Genetics" patented technology

The invention provides a method of RNA interference, which comprises contacting the cell with a fusion protein-double stranded RNA complex, the complex comprising the double stranded RNA segment containing a double stranded RNA of interest and a fusion protein, the fusion protein comprising (1) a targeting moiety, which will specifically binds to a site on a target cell, and (2) a binding moiety, which will bind to the double stranded RNA, wherein the double stranded RNA segment initiates RNA interference in the cell.

The invention discloses a CRISPR-Cas12a detection primer group for francisella tularensis and an application thereof. The invention provides the primer group for detecting francisella tularensis and is composed of an RPA primer pair and crRNA; the RPA primer pair is designed according to the nucleotide sequence shown in SEQ ID NO.1 in the francisella tularensis genome; the crRNA comprises an anchoring sequence and a guide sequence, wherein the anchoring sequence can be combined with a cas protein, and the guide sequence is matched with an amplification product sequence of an RPA primer pair. The detection primer group has the characteristics of short detection time, high amplification efficiency, high sensitivity, strong specificity and the like, and the detection sensitivity on francisella tularensis positive plasmids can reach 900 copy/ml, and the detection sensitivity on the francisella tularensis genome can reach 1260 fg/mL. The francisella tularensis onsite diagnosis method has awide application prospect.

The invention belongs to the technical field of biology, and particularly relates to a characteristic polypeptide for identifying the deer-horn glue of Cervus nippon or Cervus elaphus, and application of the characteristic polypeptide. The characteristic polypeptide provided by the invention is as follows: a peptide fragment 1 is as shown in SEQ ID No. 1: ASTGAALVVR; and a peptide fragment 2 is as shown in SEQ ID No. 2: TPVGQGPSVPVPNR. The characteristic polypeptide and the detection method provided by the invention provide reference for searching a characteristic peptide fragment in related species lacking in a database. The characteristic polypeptide provided by the invention has excellent specificity and stability for the deer-horn glue of the Cervus nippon or the Cervus elaphus, is high in specificity, can be used for identifying deer-horn glue medicinal materials and Chinese patent medicines added with the the deer-horn glue medicinal materials, and has a good application prospect.

The invention discloses a drought-resistant related SSR sequence derived from abnormal cotton and application thereof. The invention provides application of a specific DNA fragment or related biomaterials thereof in cultivation of drought-resistant cotton varieties or strains or improvement of the drought resistance of cotton. The specific DNA fragment is a fragment located on chromosome 5 of abnormal cotton and at least containing a sequence from an SSR molecular marker JAAS6365 (SEQ ID No. 1) to an SSR molecular marker JAAS5604 (SEQ ID No. 2). The related biomaterials are a vector, an expression cassette, recombinant bacteria or transgenic cell line containing the specific DNA fragment. The chromosome fragment provided by the invention has important application value in drought-resistantcotton breeding, and at the same time the SSR molecular marker developed by the invention provides a molecular basis for cultivation of drought-resistant cotton varieties that can be applied to production.

The invention provides oligonucleotides and their complements that can be used as allele-specific probes or primers for sequencing, oligonucleotide probe hybridization, and allele-specific amplification. Such oligonucleotides can be used, for example, to facilitate genetic distinction between individual plants in plant populations.

The invention discloses a construction method of a sequencing library. The construction method comprises the following steps: providing a primer sequence fragment for carrying out amplified library construction on desoxyribonucleic acid, and carrying out treating to obtain an amplification primer with G at a 5' terminal, wherein the treating comprises the steps: connecting the G at the 5' terminal if the basic group at the 5' terminal is not G; connecting the G at the 5' terminal or not if the basic group at the 5' terminal is not G; carrying out cyclic amplification by adopting an amplification primer with G at the 5' terminal to obtain an amplified fragment with C at a 3' terminal; adding A to a 3' terminal of the deoxyribonucleic acid; and connecting a joint containing a T sticky terminal with the amplified fragment with the A at the 3' terminal to generate a connected product. The invention also discloses an amplification primer for constructing the sequencing library. The invention also discloses the sequencing library. The invention also discloses a kit for constructing the sequencing library, a mutant site detection kit, a chromosome ploidy detection kit and a gene fusion detection kit. The invention also discloses a sequencing method.

The invention relates to an SSR (Simple Sequence Repeat) marker primer for black camellia hybrid identification. The primer has a primer number SSR31, an upstream primer: 5 '-CAAGGCAGAGTAGGCTGAGG-3', a downstream primer: 5 '-AAAAGGAAGGGAAAGTCA-3', a repetitive unit: (AGC) 7 and a fragment size of 212-273; the primer number is SSR48, the upstream primer is 5 '-GTTGGAGGTTTTTCAGCCAC-3', the downstream primer is 5 '-ATTGAAAGTCGCTGCTTCGT-3', the repetitive unit is (CCG) 7, and the fragment size is 249 to 300; the primer number is SSR250, the upstream primer is 5 '-ACCTCAGCTTCCATAGA-3', the downstream primer is 5 '-ATCTTGACGGGCGTAACATA-3', the repetitive unit is (CCG) 5, and the size of the fragment is 261 to 315. The invention also discloses a method for detecting the content of the CCG in the sample. The SSR marker disclosed by the invention is good in repeatability and strong in specificity; the black camellia hybrid is effectively identified by using an SSR molecular marker technology, the relationship between the black camellia hybrid and parent heritable variation is defined, and rapid identification and early selection of the black camellia hybrid offspring are realized.

The invention provides a primer group, kit, system and method for RET (rearranged during transfection) gene mutation detection. The primer group includes at least one of five primer pairs to amplify exons 10, 11, 13 to 16 of RET gene; nucleotide sequences of upstream and downstream primers in the five primer pairs are shown as SEQ ID NO. 1 to 10. Upon multiple PCR (polymerase chain reaction) detection with the primer group, all loci of the exons 10, 11, 13 to 16 of the RET gene can be subjected to mutation detection, and detection time can be shortened effectively.