Primer group, kit, system and method for RET (rearranged during transfection) gene mutation detection

[0049] The preparation method of embodiment 1 DNA sample to be tested

[0050] Oral exfoliated cells collected with buccal swabs or collected fresh peripheral blood samples were extracted with Tiangen Buccal Swab Genomic DNA Extraction Kit (DP322) or Blood / Cell / Tissue Genomic DNA Extraction Kit (DP304), and used NP80-touch (IMPLEN, Germany) measures the concentration and purity of DNA, and then preserves the genomic DNA.

[0051] Embodiment 2 RET gene mutation detection

[0052] According to literature research, select exons No. 10, 11, 13, 14, 15, and 16 of the RET gene, search the DNA sequence of each exon through NCBI, design and synthesize the amplification primers of the embodiments of the present invention, and design primers. In other words, online / offline databases or software such as PrimerQuest, Primer Premier 5.0, and DNAMAN can be used to design primers and analyze dimers, stem-loop mismatches, etc., to ensure that the amplified fragments will not interact with each other to produce non-specific products. Or dimer, primers are designed at both ends of the gene mutation site, and the annealing temperature of the five primer pairs is basically the same. Then, it was diluted to a desired concentration such as 10 μM as needed. The nucleotide sequences and product sizes of the amplification primers of the embodiments of the present invention are shown in Table 1. The primer set provided i