The invention belongs to the field of detection of chromosomes, and particularly relates to a human
chromosome 10
centromere probe and a preparation method and kit thereof. A
nucleotide sequence of the human
chromosome 10
centromere probe is shown as by SEQ ID NO.1. The human
chromosome 10
centromere probe provided by the invention is designed for a high-
repeatability and high-specificity region of a human chromosome 10 centromere region, and has a relatively good recognition effect on a human chromosome 10 centromere.