Electrochemical immunosensor of electroactive substance modified MOF composite material as well as preparation and application of electrochemical immunosensor

An immunosensor and composite material technology, applied in the field of metal organic framework materials and biosensors, can solve the problems of low antibody and signal label loading, unfavorable signal formation and amplification, increase analysis time and cost, and simplify the immune probe. Labeling process, low cost, low toxicity effect

Active Publication Date: 2021-06-15
SHANTOU UNIV
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Problems solved by technology

However, it is worth noting that electrochemical immunosensors for the simultaneous determination of multi-component markers still have some deficiencies.
First, most of the reported electrochemical methods require expensive and complex signaling molecule labeling processes, which limits the development of multicomponent electrochemi

Method used

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  • Electrochemical immunosensor of electroactive substance modified MOF composite material as well as preparation and application of electrochemical immunosensor
  • Electrochemical immunosensor of electroactive substance modified MOF composite material as well as preparation and application of electrochemical immunosensor
  • Electrochemical immunosensor of electroactive substance modified MOF composite material as well as preparation and application of electrochemical immunosensor

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Experimental program
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Embodiment approach 1

[0044] 1. A preparation method of an electrochemical immunosensor modified with an electroactive substance MOF composite material, mainly comprising the following steps:

[0045] (1) Synthesis of nanoparticles / reduced graphene composite (Au / rGO)

[0046] Graphene oxide (GO) is synthesized by the Hummers method: take 1.0g natural flake graphite powder, 0.5g NaNO 3 and 23.0 mL of 98% H 2 SO 4 React in ice bath for 30min; slowly add 3.0g KMnO 4 , the temperature was controlled below 20°C to continue the reaction for 2 hours, then slowly raised to 35°C, and kept at a constant temperature for 30 minutes; slowly added 46.0 mL of water, heated to 98°C, and kept at a constant temperature for 15 minutes to ensure complete oxidation of graphite. After the reaction, remove the heat source and cool in a water bath for 10 minutes; take 140.0 mL of water to dilute the reaction mixture, and then add 3.0 mL of 30% H 2 o 2 , the mixture was bright yellow. When the reaction solution was

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Abstract

The invention relates to an electrochemical immunosensor of an electroactive substance modified MOF composite material as well as preparation and application of the electrochemical immunosensor. According to the electrochemical immunosensor, a Cu < 2 + > modified metal-organic framework composite material and a [Fe (CN) 6] < 3-> modified metal-organic framework composite material are used as signal tags for simultaneously detecting multiple components; and the immunosensor platform is prepared from the Au/rGO composite material. The electrochemical immunosensor provided by the invention has the advantages of wide linear range, high sensitivity, good selectivity, greenness, simplicity and the like, provides an effective method for simultaneously detecting two biomarkers of acute myocardial infarction (AMI) in clinical serum, has certain application potential in the aspect of early accurate diagnosis of AMI, and has a bright application prospect; and the method has enlightening significance on detection of other related biomarkers.

Description

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Claims

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Application Information

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Owner SHANTOU UNIV
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