5 results about "Immunofluorescence" patented technology

The invention discloses application of bitter gourd exosomes in radiation heart injury protection, the bitter gourd exosomes and rat myocardial cells (H9C2) are co-cultured and subjected to radiation treatment, MTT experiments show that 5-25 [mu] g/ml of the bitter gourd exosomes can promote the cell viability of the radiated H9C2 myocardial cells, and 10 [mu] g/ml of the bitter gourd exosomes are selected as the use concentration of the bitter gourd exosomes. Under the condition that the bitter gourd exosome is 10 [mu] g/ml, Ki-67 fluorescent staining proves that the bitter gourd exosome can promote proliferation of radiated H9C2 myocardial cells. An Annexin V-PE/7-AAD flow apoptosis experiment and an apoptosis protein cleaved-caspase3 immunoblotting test prove that the bitter gourd exosome can be used for reducing the apoptosis of H9C2 myocardial cells after radiation. Immunofluorescence staining and protein immunoblotting of a DNA damage marker gamma-H2A. X show that the bitter gourd exosome can reduce DNA damage of H9C2 myocardial cells after radiation.

The invention relates to an independent multichannel immunofluorescence microfluidic chip and an immunofluorescence detection method. The independent multi-channel immunofluorescence microfluidic chipcomprises at least two independent channels, each channel comprises an equivalent uniform flow channel, a filtering area, a buffer area, a fluorescence coating area, a time control valve, a detectionarea and a waste liquid area which are connected in sequence, and the equivalent uniform flow channels of all the channels are connected with the same liquid inlet. According to the independent multi-channel immunofluorescence microfluidic chip, liquid is equally divided in the sample feeding stage, different channels have independent fluorescence antibody coating areas, space-time valves and independent waste liquid areas, and the problem that different detection items may cause mutual interference is solved; each independent channel can integrate a plurality of detection items which do notinterfere with one another, so that the detection precision and efficiency are improved and the cost is reduced while high flux is realized.

The invention discloses a preparation method and application of a fluorescent reagent strip for quantitatively detecting the concentration of ProAKAP4, and belongs to the field of in-vitro diagnostic reagents; a fluorescence immunochromatography technology is introduced into the detection of proAKAP4, and the prepared immunochromatography test strip for proAKAP4 adopts a double-antibody sandwich method; a proAKAP4 antigen in a sample pad is combined with a proAKAP4 antibody marked by fluorescent microspheres on a marking pad under the chromatographic action to form a compound, the compound moves to a detection line of a coating film under the chromatographic action, and the detection line of the coating film is coated with an antibody for identifying the other epitope of the proAKAP4 antigen to form a double-antibody sandwich compound; proAKAP4 with the concentration as low as 0.1 ng/mL in a seminal fluid sample can be detected, a detection result can be read through an immunofluorescence analyzer, professional operators are not needed, single-person quantitative detection of proAKAP4 is achieved, and the kit has the advantages of being convenient and rapid to use, easy to operate, high in sensitivity, easy to operate, low in detection cost and the like.

The invention belongs to the field of genetic engineering and discloses a genetic engineering strain for displaying Escherichia coli enterotoxin by Saccharomycetes and application of the genetic engineering strain. Fusion genes of Escherichia coli heat-resistant enterotoxins (ST) estA and estB after site-directed mutagenesis are connected into a surface display plasmid pYD1, the surface display plasmid pYD1 is converted into saccharomyces cerevisiae through electric shock, an ST-yeast genetic engineering strain is obtained through screening, and it is proved through immunofluorescence detection that an ST-fused protein is successfully displayed on the surface of the saccharomyces cerevisiae. Animal experiments prove that the engineering strain not only has a good probiotic function of the saccharomyces cerevisiae and can be used for improving the immunity of intestinal mucosa, but also can be used for effectively preventing animal intestinal flora imbalance and small intestinal mucosa structure damage caused by enterotoxigenic Escherichia coli, and has a specific immunoprophylaxis function on diseases such as animal diarrhea caused by the enterotoxigenic Escherichia coli.