The invention discloses an application of 6-Thioinosine in inhibition of ZIKV (Zikov Institute of Kovary) replication. The invention finds that 6-Thioinosine can inhibit the replication of
Zika virus in cells. According to the invention, an
enzyme-linked immunosorbent
assay is adopted to obtain that the EC50 value of 6-Thioinosine for inhibiting the replication of ZIKV in the cells is 18.82 + / -4.66 [mu] M. According to the invention, the EC50 value of 6-Thioinosine for inhibiting the replication of ZIKV in the cells is 18.82 + / -4.66 [mu] M; the EC50 value of the 6-Thioinosine for inhibiting the replication of ZIKV in cells, which is obtained by adopting an
indirect immunofluorescence experimental method, is 16.69 + / -7.89 [mu] M; the
cytotoxicity of the 6-Thioinosine is detected by adopting a CCK-8 method, the CC50 value is 112.2 [mu] M, and the SI index (CC50 / EC50) is 5.962. The invention provides a scientific basis for research and development of medicines for effectively inhibiting ZIKV.