Carrier and testing method

[0302]The testing method of the present embodiment includes a process (an extraction efficiency calculation process) of calculating the extraction efficiency of the nucleic acid from the cell A using the carrier.

[0303]In addition to the above process, the testing method of the present embodiment preferably further includes a process (a determination process A) of determining the accuracy of the measurement value of the gene by a genetic testing apparatus from the extraction efficiency.

[0304][Extraction Efficiency Calculation Process]

[0305]In the extraction efficiency calculation process, first, a nucleic acid is extracted from cell A using a carrier. Then, quantitative PCR is performed using the obtained nucleic acid to quantify the copy number of the nucleic acid. Next, the value obtained by dividing the quantification value by the known copy number of the nucleic acid present on the base material is calculated in terms of percentage as the extraction efficiency of the nucleic acid.

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[0317]In the case where the genetic testing apparatus is a quantitative PCR apparatus, the testing method of the present embodiment include a process (an extraction efficiency calculation process) of calculating the extraction efficiency of the nucleic acid from the cell A using the carrier; a process of amplifying the nucleic acid with a quantitative PCR apparatus by using two or more carriers respectively having different total copy numbers of nucleic acids contained in the carrier, creating a calibration curve, and then calculating the amplification efficiency (an amplification efficiency calculation process); and a process of determining the accuracy of the quantification value of the gene, where the quantification value is obtained with the quantitative PCR apparatus, from the extraction efficiency and the amplification efficiency (a determination process B).

[0318]In addition to the above process, the testing method of the present embodiment preferably further includes a process (

[0332](Confirmation Test for Extraction Efficiency of Nucleic Acid from Water-Decomposable Carrier)

[0333]1. Preparation of Sample

[0334]A sample was prepared by applying 500 yeast cells containing one copy of a nucleic acid (6203-a-G (GenBank accession number: AB610938.1)) on a water-decomposable carrier (trade name “120MDP”, manufactured by Nippon. Paper Papylia Co., Ltd.,) using inkjet technique.

[0335]2. Nucleic Acid Extraction

[0336]Then, 0.4 U / 4 μL of a yeast cell wall digesting enzyme (trade name “Zymolyase-100T”, manufactured by Nacalai Tesque, Inc.) was added dropwise onto a base material made of a water-decomposable material and incubated at 37° C. for 30 minutes. After incubation, a portion at which cells were supported was cut out to a diameter of 6 mm with a biopsy trepan. The cut-out water-decomposable carrier was inserted in a 1.5 mL microtube with forceps, 150 μL of UltraPure Distilled Water (manufactured by Thermo Fisher Scientific, Inc.) was added thereto, and the resul