6 results about "Library" patented technology

The invention discloses a construction method of a sequencing library. The construction method comprises the following steps: providing a primer sequence fragment for carrying out amplified library construction on desoxyribonucleic acid, and carrying out treating to obtain an amplification primer with G at a 5' terminal, wherein the treating comprises the steps: connecting the G at the 5' terminal if the basic group at the 5' terminal is not G; connecting the G at the 5' terminal or not if the basic group at the 5' terminal is not G; carrying out cyclic amplification by adopting an amplification primer with G at the 5' terminal to obtain an amplified fragment with C at a 3' terminal; adding A to a 3' terminal of the deoxyribonucleic acid; and connecting a joint containing a T sticky terminal with the amplified fragment with the A at the 3' terminal to generate a connected product. The invention also discloses an amplification primer for constructing the sequencing library. The invention also discloses the sequencing library. The invention also discloses a kit for constructing the sequencing library, a mutant site detection kit, a chromosome ploidy detection kit and a gene fusion detection kit. The invention also discloses a sequencing method.

The invention provides a high-throughput detection method of an R ring. The high-throughput detection method comprises the following steps: firstly, closing free 3'-OH of DNA in a sample by using ddNTP; then, converting RNA in the sample into the DNA with biotin modification, and then performing DNA fragmentation and terminal repair; connecting joints; enriching the DNA with biotin modification by streptavidin magnetic beads; and performing chain-specific library amplification and sequencing. The invention provides a simple, convenient and rapid technology for detecting the R ring in the genome range, the R ring can be efficiently and accurately identified, and the whole process is simple to operate, short in time consumption (not more than 4 hours), wide in applicability, high in sensitivity and accuracy and suitable for development and application of R ring detection commodity kits.

The invention provides a phagemid vector construction method for constructing a phage antibody library and an antibody screening method. Phagemids are filamentous phagemids containing restriction enzyme cutting sites of bovine thrombin between coat protein PIII coding genes and target gene insertion sites. The restriction enzyme cutting sites of bovine thrombin are introduced into the phagemids, and hydrolytic cleavage of protein is carried out, so that the background in which the phagemids are non-specifically eluted is reduced. By using the phagemids of the present invention, the antibody screening efficiency is higher, and the problem of low screening efficiency in a phage display technology in the prior art is solved.

The invention relates to a DNA library for detecting and diagnosing corneal dystrophy disease-causing genes as well as application of the DNA library. The library comprises 24 disease-causing genes related to corneal dystrophy; 24 corneal dystrophy disease-causing genes are preferably selected, a probe pool is designed, a target area library aiming at the 24 corneal dystrophy disease-causing genesis established, and the library performs sequencing by a high-throughput sequencing technology, looks for disease-cause mutation and provides genetic and molecular biological basis for clinical diagnosis. The library has the characteristics of accuracy, rapidness, flexibility and low cost; 24 gene detection areas can detect various kinds of common corneal dystrophy such as epithelial and subepithelial corneal dystrophy, Bowman's membrane corneal dystrophy, stromal corneal dystrophy, descemet's membrane and corneal endothelium dystrophy; and important significance and clinical value in diagnosis and differential diagnosis on the corneal dystrophy are achieved.