Method of preparing chromatographic materials

一种吸附剂、单乙烯的技术，应用在制备新的树脂用于高选择性色谱层析领域，能够解决材料损失大、产品成本高、产物收率低等问题

Inactive Publication Date: 2016-07-20
NEW PROTEINTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the disadvantages of the existing chromatographic material application are: a multi-step purification structure with pretreatment operations is required, the material loss during the purification process is large, and the yield of the obtained product is low, and the product cost is high
Therefore, in terms of commercial yield, only less than 50% of the original IgG antibody can be obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0075] The 250 ml flask was equipped with a stirrer, an argon inlet tube and a dropping funnel. The flask was poured with 120 mL of 20% sodium sulfate and bubbled with argon for 20 minutes. At the same time, 2 ml of methyl phenylacrylate, 3 ml of methacrylic acid, 0.921 g of HTA, 2 ml of alcohol laurate, 11 ml of butanol, 11 ml of cyclohexanol were added. After the comonomer is dissolved, 0.06 g of 1,1'-azocyclohexane is added. The polymerization reaction was carried out at a temperature of 60° C. for 10 minutes to obtain a viscous prepolymer. The prepolymer was dispersed into 120 ml of 20% sodium sulfate. The temperature was maintained at 60°C to 70°C for 30 minutes. The temperature was then raised to 100°C and the reaction was continued at this temperature for 1 hour. In the next step, the resulting spherical particles are separated from the dispersion after cooling to a temperature below about 25°C. The particles were then washed with glacial acetic acid, then water, t...

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PUM

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Abstract

The invention relates to a sorbent material. The sorbent material comprises a plurality of cross linked monovinyl monomers defining a matrix, in a ratio of the volume of hydrophobic monovinyl monomers to hydrophilic monovinyl monomers of approximately 5:95 to approximately 40:60, the matrix being bufferable to pH ranges from approximately 5 to approximately 9, and, of particle sizes between approximately 10 micrometers to approximately 300 micrometers. The sorbent is used in chromatographic columns to promote binding of Immunoglobulin G (IgG) from blood plasma, for its isolation, such that the isolated IgG is then extracted from the sorbent. The sorbent is also used in chromatographic columns to promote binding of Immunoglobulin G (IgG) monoclonal antibodies from transgenic milk, for their isolation, such that the isolated IgG monoclonal antibodies are then extracted from the sorbent.

Description

[0001] Cross References to Related Applications [0002] This case cites U.S. Patent Provisional Application No. 61 / 905,238 filed on November 17, 2013, titled "Method for Preparing Novel Chromatographic Materials and New Chromatographic Layers for Highly Selective Chromatography" Analytical Materials"; and U.S. Patent Provisional Application No. 61 / 905,239, filed November 17, 2013, entitled "Methods for Preparing Hybrid Resins and Hybrid Resins for High Selectivity Chromatography" ”, which is incorporated herein in its entirety by reference and claiming priority to the above application. technical field [0003] The present invention relates to a method for preparing novel resins for high-selectivity chromatography. Background technique [0004] The global demand for plasma-based products is increasing, so efficient and economical methods are sought for biotechnological purification of products. Included in the above methods is the purification of various proteins, enzymes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06B01J20/285B01D15/26B01D15/38C07K1/16
CPCB01J20/285C07K1/16C08F220/06B01D15/3847B01D15/327B01D15/362B01D15/424B01J20/28004B01J20/28085B01J20/267B01D15/361
Inventor 卡瑞纳·罗兹伊斯卡
Owner NEW PROTEINTECH
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