Novel method for purifying arsenic chelating type immune complex

An immune complex and chelating technology, applied in the field of immune complexes, can solve the problems of increased purification and purification time, limited use range, long purification time, etc., and achieves reduction of purification cost, time, and working environment. less demanding effects

Inactive Publication Date: 2018-12-14
广东腾湃医疗股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this new method described by this inventor lies within its ability to make it easier for users who want more efficient ways to clean up their equipment than current methods that require them themselves or other suppliers' products. This reduces costs while still effectively removing impurities from these systems.

Problems solved by technology

The technical problem addressed in this patented text relates to how efficiently cleaning or separating an immunological compound from other substances that may be harmful when used with patients due to its potential negative effects caused by exposure thereto.

Method used

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Embodiment Construction

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0017] The invention provides a technical solution: a novel method for purifying arsenic chelated immune complexes, comprising the following steps:

[0018] Step 1: first prepare the chelating agent solution, and then dissolve the chelating agent in the solvent to obtain the chelating agent solution;

[0019] Step 2: Take an appropriate amount of carrier protein, and add the carrier protein into the borate buffer solution to prepare the carrier protein solution;

[0020] Step 3: Add the prepared chelating agent solution into the carri

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Abstract

The invention discloses a novel method for purifying an arsenic chelating type immune complex. The method comprises steps as follows: S1, a chelating agent solution is prepared firstly through dissolution of a chelating agent in a solvent; S2, a proper amount of carrier protein is taken and added to a borate buffer solution, and a carrier protein solution can be prepared; S3, the prepared chelating agent solution is added to the carrier protein solution, the carrier protein solution is put in a constant-temperature water bath and stirred uniformly for constant-temperature water bathing, and amixed solution can be obtained; S4, a semipermeable membrane is homemade; S5, arsenic ions and other heteroions which are not chelated are removed through pre-purification; S6, unbound carrier proteinis removed. Part of articles are homemade in the purification process, so that the purification cost can be notably reduced, the requirements for working environments are lower, the applicable rangeis wider, the purification time is effectively shortened by use of a combined chromatographic column, and the cost is reduced to a certain extent.

Description

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Claims

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Application Information

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Owner 广东腾湃医疗股份有限公司
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