Preparation and identification method of taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine

A technology for gene recombination and Taenia solium, which is applied in the field of preparation and identification of Taenia solium TSOL18 gene recombinant BCG vaccine, can solve problems such as no research on the recombinant BCG vaccine of Taenia solium, and achieves the advantages of being suitable for mass production, easy to preserve, huge economical and economical. The effect of social benefit

Inactive Publication Date: 2015-04-08
ZUNYI MEDICAL UNIVERSITY
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AI Technical Summary

Benefits of technology

This patented technology allows researchers to create an improved version or modified form (recombi) of bacteria called Bacillus thuringiensis(BTC). These microorganisms are able to kill harmful insects such as ticks on crops without causing damage during their growth process. They also work alongside other living organism like yeast cells when they infect them. By adding these genes together, we could make better ways to protect plants against diseases caused by parasites.

Problems solved by technology

This patented technical solution describes how certain genes can help protect against various types of infectious agents like protozoa or helminths from humans that cause malaria. These proteins may play key roles during this process due to its ability to stimulate specific defense mechanisms such as phagocytose killing cells called neutrophils.

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  • Preparation and identification method of taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine
  • Preparation and identification method of taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine
  • Preparation and identification method of taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine

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Embodiment Construction

[0032] The preparation and identification of the Taenia suis TSOL18 gene recombinant BCG vaccine of the present invention comprises the following steps:

[0033] (1) Amplification and identification of plasmid pMV261: first prepare E.coli DH5α competent cells, use the purchased pMV261 plasmid to transform E.coli DH5α competent cells, further amplify and identify, and further culture the correctly identified pMV261 plasmid, Save the seeds and extract the plasmids for later use.

[0034] (2) Construction and identification of recombinant plasmid pMV261-TSOL18: The prepared and preserved vector pGEX-1λT-TSOL18 and plasmid pMV261 were digested with BamHI and EcoRI respectively, and the digested products were subjected to 1% agarose gel electrophoresis, washed with clean Cut the agar block containing the DNA to be recovered under ultraviolet light with a scalpel, put it into a 1.5 ml centrifuge tube of known weight, recover and purify the PCR product. Then the corresponding fragments

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Abstract

The invention discloses a preparation and identification method of taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine. The preparation and identification method is characterized by comprising the following steps: (1) amplification and identification of plasmid pMV261; (2) construction and identification of recombinant plasmid pMV261-TSOL18; and (3) construction and identification of the taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine, namely 1) preparation of BCG competent cells, 2) electrotransformation of the BCG competent cells by use of the recombinant plasmid pMV261-TSOL18, 3) identification of the taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine, namely picking up individual bacterial colony in the positive colonies to an M7H9ADC liquid culture medium containing Kana for shaking culture, taking the bacterial liquid for centrifuging; abandoning the liquid supernatant, washing by use of ddH2O, centrifuging and abandoning the liquid supernatant, adding ddH2O, and re-suspending before boiling water bath, putting in ice bath, centrifuging, and absorbing the liquid supernatant as a PCR template for PCR identification.

Description

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Claims

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Application Information

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Owner ZUNYI MEDICAL UNIVERSITY
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