3 results about "Chlorogenic acid" patented technology

The invention discloses a rapid and high efficiency chlorogenic acid and galuteolin in honeysuckle flower extraction method and a HPLC quantitative analysis method, and which comprise the following steps: superhigh pressure extraction and quantitative analysis. Superhigh pressure extraction comprises the steps as follows: firstly crushing dried honeysuckle flower and filtering by a sieve with 60-80 meshes, adding water or ethanol, methanol and n butanol organic solvent into the honeysuckle flower powder according to 1:10-1:100 solid liquid ratio, mixing and sealing, immersing at normal temperature, placing the mixture into a high pressure container, leaching with pressure at 20-60 DEG C. for 1-5 min, relieving pressure, carrying out centrifugation of extract product and obtaining the honeysuckle flower extract. Quantitative analysis: after centrifugation, diluting 2ml of the obtained supernatant to 10 ml with distilled water in a volumetric flask, refrigerating for standby. The chlorogenic acid and galuteolin in honeysuckle flower are extracted by a superhigh pressure technology and are carried out by a content determination, and the invention has the characteristics of high recovery rate, short extraction time, small solvent amount, simple operation, accuracy, good repeatability, no pollution, safety, low energy consumption, etc.