Detection method for content of chlorogenic acid in stauntonia chinensis DC
一种检测方法、绿原酸的技术,应用在及生物领域,能够解决测定效果不准确、浓度低等问题,达到避免再生处理操作、检测结果准确、检测方法简单的效果
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[0018] The following is further described in detail through specific implementation methods:
[0019] 1 Materials and methods
[0020] 1.1 Materials and reagents
[0021] Chlorogenic acid standard was purchased from Aladdin Company, potassium dihydrogen phosphate (KH 2 PO 4 ), phosphoric acid were purchased from Guangzhou Guanghua Technology Co., Ltd.; methanol and acetonitrile were purchased from Aladdin Company; the water used was ultrapure water, which was made by our laboratory.
[0022] 1.2 Instruments and equipment
[0023] Waters H-Class ultra-high performance liquid chromatography (equipped with Waters PDA detector, empower chromatography management system); METTLER TOLEDO ten-thousandth balance; METTLER TOLEDO pH meter; eppendorf pipette gun; ELGA Chorus ultrapure water treatment system; Ningbo Xinzhi ultrasonic cleaning instrument.
[0024] 1.3 Method
[0025] 1.3.1 Chromatographic conditions
[0026] Thermo Fisher Accucore aQ (150x3mm, 2.6μm) chromatographic...
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