6 results about "Fluorescein" patented technology

The invention relates to an MLDH (magnetic layered double hydroxides)-Fluorescein intercalation assembled type fluorescent probe. The invention is characterized in that the MLDH-Fluorescein intercalation assembled type fluorescent probe is prepared by a low-temperature ion exchange reaction, with MLDH as carriers and fluorescein as an intercalation object. The synthesized MLDH-Fluorescein probe has superparamagnetism, bright fluorescence signals, high thermal stability, strong nuclear targeting property in cell transmission, and capability of quickly infiltrating a cell membrane and passing through cytoplasm to deliver a fluorescent material to cell nucleus and enrich the fluorescent material to a nucleolus, so that the MLDH-Fluorescein probe can be used as a fluorescent probe for researching a cell transport mechanism of an MLDH system and evaluating the nuclear targeting transmission efficiency. The MLDH-Fluorescein intercalation assembled type fluorescent probe is applicable to establishment of a fluorescein, fluorandiol marked medicament or biomolecular targeting transport system and has the advantages of bright fluorescence signals, high storage stability, integration of multiple functions, simplicity in operation, low cost and the like.

The invention discloses a permanent magnet synchronous motor model prediction speed control method using firefly group optimization, which comprises the following steps: updating fluorescein, finding a better position in a decision radius, calculating the probability that each position is selected, salculating a moving step, updating the position of the firefly, setting the maximum number of iterations, and updating the decision domain range. The invention further discloses a permanent magnet synchronous motor model prediction speed controller using firefly group optimization, and the controller comprises a reference track module, a rolling optimization module, a feedback correction module and a prediction model module. The optimal weight coefficient can be obtained under different working conditions, so that high-performance speed control is obtained. Simulation and experiments show that the problem that the weight coefficient of the controller is difficult to adjust and the problem that the performance of the controller is poor due to fixed parameters are solved, and the controller has good dynamic response capability and robustness due to the real-time change of the weight coefficient.

The invention relates to the technical field of immunodetection analysis, in particular to a porcine reproductive and respiratory syndrome virus antibody detection kit and application thereof. The kit takes an anti-fluorescein FITC antibody labeled magnetic particle solution, a fluorescein FITC labeled porcine reproductive and respiratory syndrome virus antigen solution, a marker, a quality control product, a luminous substrate, a buffer solution and a concentrated washing solution as components. The magnetic particles are used as carriers, so that the coating surface area and the effective coating amount of the antibody are increased, the antibody is saved, and the establishment of a relatively wide detection range is facilitated; meanwhile, an anti-fluorescein FITC antibody coupling magnetic bead is adopted to increase the antibody binding rate, so that the binding with a fluorescein-labeled biological ligand is quicker and more accurate, and non-specific binding is well reduced; according to the invention, the chemiluminescence detection technology with high sensitivity and the immunoreaction with high specificity are combined, the operation is simple, the consumed time is short, long-time color development and termination are not needed, the sensitivity is high, the specificity is good, and the porcine reproductive and respiratory syndrome virus antibody can be rapidly, simply and sensitively detected.