Porcine reproductive and respiratory syndrome virus antibody detection kit and application thereof

[0019] The anti-fluorescein FITC antibody-labeled magnetic microparticle solution, wherein the anti-fluorescein FITC antibody is coupled with the anti-FITC antibody by a two-step glutaraldehyde method, and contains 0.5% BSA, 1% casein, The blocking was completed with phosphate buffer at pH 7.2 (0.02 mol / L).

[0020] The preparation of the fluorescein FITC-labeled porcine reproductive and respiratory syndrome virus antigen solution includes the following steps:

[0021] S1: Mix FITC and porcine reproductive and respiratory syndrome virus at 1:1, and react at 37°C in the dark for 12 hours to form a labeling solution.

[0022] S2: dialyze the labeled solution obtained in S1 with 0.01MPBS at 4°C for 24h, add an equal volume of glycerol, and store at -20°C.

[0023] The present invention also provides an application of the porcine reproductive and respiratory syndrome virus antibody detection kit, characterized in that it includes the following steps:

[0024] S1: Put the sample r

[0032] specific experiment

[0033] Three batches of prepared ASFV antibody magnetic particle chemiluminescence kits were used to detect ASFV antibody positive serum (20); ASFV antibody negative serum (15); swine fever virus positive serum 5; African swine fever virus positive serum 5; 5 Seneca virus positive sera; 5 foot-and-mouth disease virus O-positive sera; 5 foot-and-mouth disease virus A-positive sera; porcine vesicular virus positive sera (5); a total of 65 sera were tested, according to the measured luminescence value Calculate S / N to determine negative and positive.

[0034] Table 1 Specificity test of porcine reproductive and respiratory syndrome virus antibody detection kit

[0035]

[0036]

[0037] The results showed that the "Swine Reproductive and Respiratory Syndrome Virus Antibody Detection Kit" did not cross-react with the positive serum of swine fever, porcine reproductive and respiratory syndrome, Seneca, foot-and-mouth disease type O, foot-and-mouth

[0039] Sensitivity test

[0040] Dilute the positive standard serum with the sample diluent at 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256, 1:512, 1:1024, It was diluted at a ratio of 1:2048 and used as a sensitive quality control serum for detection with three batches of ASFV magnetic particle chemiluminescence kits prepared.

[0041] Table 2 Sensitivity test of porcine reproductive and respiratory syndrome virus antibody detection kit

[0042]

[0043]

[0044] The test results showed that the data of the three repeated experiments were consistent, and the minimum detection amount of porcine reproductive and respiratory syndrome antibody could reach 1:256.