13 results about "Fluorescence intensity" patented technology

The invention discloses preparation of fluorogold nano-clusters and application of the fluorogold nano-clusters to tetracycline and copper fluorescent probes. The fluorogold nano-clusters (Au NCs) aresynthesized with a one-step method from casein separated from milk and carbon quantum dots as a stabilizer and a reducer for synthesis of the Au NCs. The Au NCs perform emission three times at 433 nm, 702 nm and 1052 nm under the excitation wavelength of 350 nm, tetracycline has a linear quenching effect at 702 nm, Cu<2+> is added to an Au NCs system after quenching, fluorescence recovers, and fluorescence intensity at 1052 nm is almost not affected in the overall process; tetracycline is added to the Au NCs, observation is carried out under a UV (ultraviolet) lamp with wavelength of 365 nm,red fluorescence of the Au NCs changes into yellow fluorescence, and after addition of Cu<2+>, the yellow fluorescence disappears gradually and the red fluorescence recovers gradually. Therefore, a near-infrared ratio fluorescence switch for tetracycline and Cu<2+> and UV visual dual-signal detection are established. The method has the advantages of adopting a fluorescence spectrum in a near infrared region and being small in interference, high in specificity and detection sensitivity, convenient to operate and capable of detecting tetracycline and Cu<2+> simultaneously.

The invention provides a method for rapidly optimizing metal ions to promote bacillus to produce spores by a response surface method. The method comprises the following steps: taking spores DPA fluorescence intensity as a response value; screening metal ions which possibly affect fermentation of spores by a simple factor design of experiments at first; and selecting types of metal ions which remarkably improve the fluorescence intensity and the most suitable adding concentration when the concentration of the ions is changed. A central combined design is adopted further, the fluorescence intensity of spores DPA is also used as a response value, a combined effect of the metal ions is counted, analyzed and determined through a response surface, and thus, maximization of the yield of spores under the combined condition of a certain concentration of the ions is implemented. By the method, the working strength of detection of the test design in a fermenting process of the spores can be greatly reduced, a test period is shortened, test accuracy is ensured, and the design efficiency is improved.

The invention discloses a pyridine quinoxaline double pillar [n] aromatic hydrocarbon based nanoparticle, and a preparation method and applications thereof. The preparation method comprises followingsteps: double pillar [n] aromatic hydrocarbon main body molecule H and object molecule G1 and G2 are mixed to be uniform in an organic solvent at a certain ratio, an obtained mixture is introduced into a surfactant aqueous solution so as to obtain the pyridine quinoxaline double pillar [n] aromatic hydrocarbon based nanoparticle. The pyridine quinoxaline double pillar [n] aromatic hydrocarbon based nanoparticle possesses excellent water solubility, and excellent light capturing performance; pyridine quinoxaline double pillar [n] aromatic hydrocarbon is taken as an energy donor; object moleculeG2 is taken as an energy receptor, and G1 is taken as an energy bridge, so that transmission of the subject energy to the object is realized, and the fluorescence intensity of the nanoparticle composed of H, G1, and G2 is increased to be much higher than that of G2 nanoparticle at the same concentrations.

The invention relates to a preparation method of a near-infrared light codoped silver sulfide nano-luminescence material. The preparation method comprises the steps of synthesizing a mixed precursor, synthesizing a sodium sulfide precursor, and synthesizing the near-infrared light codoped silver sulfide nano-luminescence material. The preparation method is simple, easy in operation, mild in reaction conditions, low in cost and good in operability; and the obtained Ag2S:Mn:Eu nano-luminescence material or Ag2S:Eu:Mn nano-luminescence material, the obtained Ag2S:Eu:Y nano-luminescence material or Ag2S:Y:Eu nano-luminescence material, the obtained Ag2S:Mn:Y nano-luminescence material or Ag2S:Y:Mn nano-luminescence material and the obtained Ag2S:Y:Mn:Eu nano-luminescence material or Ag2S:Y:Eu:Mn nano-luminescence material have good crystallinity and high fluorescence intensity, are uniformly and stably dispersed and have good application prospects in the aspects of sensors, the production of nano-laser materials, DNA quantitative analysis and the like.

The invention discloses a derivative based on rhodamine B and a preparation method and application thereof. The preparation method of the derivative comprises the following steps: under nitrogen protection, adding a dichloromethane solution of D-sphingol into an acetonitrile solution of N-ethyl p-toluenesulfonate of rhodamine B; in the presence of dried potassium carbonate, heating the solution tocarry out a reflux reaction to prepare a coarse product; and concentrating the coarse product and carrying out silicagel column separation to obtain a pure product. A pH titrating experiment verifiesthat in a DMSO/BR(1/99, v/v) system, the derivative does not give out fluorescence in a neutral condition, and the fluorescence intensity at 600 nm is enhanced gradually as the pH is reduced from 7.4to 2.0. The pKa value is 4.32, the pH response range is 2.0-7.4, and a golgiosome weak acidic environment is detected favorably. In addition, a probe has good selectivity, water solubility and low toxicity. An intracellular co-positioning experiment and a golgiosome pH adjusting experiment verify that the derivative can mark the golgiosome specifically in a targeted manner and can detect the pH change of the golgiosome with high sensitivity.

The invention discloses a fluorescence probe molecule as well as a preparation method and application thereof to hypochlorite ion detection. The fluorescence probe molecule is put into a testing sample with hypochlorite ions, whether the color of the testing sample is conspicuously changed or not can be detected, and in addition, the fluorescence probe molecule is good in selectivity upon hypochlorite ions and can stably respond to hypochlorous acid. The concentration of hypochlorite ions can be calculated by measuring fluorescence intensities at different wave sections, no complex physical orchemical method is implemented in the testing process, and the fluorescence probe molecule has the advantages of being simple to operate, rapid and efficient and high in sensitivity.

The invention belongs to the technical field of sperm detection, and particularly relates to a non-diagnostic quantitative detection method for active oxygen of a single sperm and application of the non-diagnostic quantitative detection method. According to the invention, a movable miniature exciting light probe is creatively used and positioned near a single sperm, and a complete detection process is constructed and comprises the steps of processing, positioning, noise reduction, measurement, baseline processing and the like. Finally, the fluorescence intensity emitted by the single sperm is accurately quantified, and the intracellular active oxygen value of the sperm can be accurately given. According to the present invention, the active oxygen detection of the single sperm level is initiated in the field, the field blank is filled, the number of the sperms is not limited, and the method can be suitable for almost all patients including severe oligospermia.

The invention provides efficient white light emission glass containing silver nanoparticles, and a preparation method thereof. The efficient white light emission glass is composed of the following components by mole: (60-x)% of SiO2, 20% of Al2O3, 20% of CaO, x% of Li2O, 0.2% of Eu2O3 and y% ofAg2O, wherein 0 =< x <= 10; and 0.1 =< y <= 4. The efficient white light emission glass containing the silver nanoparticles is obtained by the steps of preparing materials according to the above molar ratios, grinding the prepared components thoroughly to uniformly mixed; melting the mixture under an air atmosphere; and finally subjecting the melted mixture to conventional molding and annealing. The efficient white light emission glass has the characteristics of simple glass components, high fluorescent intensity, wide excitation wavelengths, and easily adjustable white light color temperature. The white light glass has the characteristics that the melting process is simple and carried out under the air atmosphere and has strong glass-forming force, low cost and no pollution to environment. The color difference caused by different luminescent lifetimes of trichromatic phosphors is solved; and at the same time, the problems of deliquesce and complex coating of conventional phosphors are prevented. The efficient white light emission glass containing the silver nanoparticles can be used for manufacturing novel white light LED illumination and display devices excited by near ultraviolet chips.