The invention relates to a detection method and detection kit for
patulin. The method comprises the following steps that (A) a
patulin aptamer (Apt) singlestrand
signal DNA (ssDNA) probe is subjected to hybridization, and a hybridization chain is formed; (B) the hybridization chain is in reaction with a to-be-tested sample, and when
patulin exists in the to-be-tested sample, the ssDNA probe is released by the reaction of the hybridization chain and the patulin; (C) the hybridization chain is changed into
double chain DNA by the aid of
DNA amplification, the
double chain DNA hydrolyzed into mononucleotide under the selective
catalysis of excision
enzyme, and the ssDNA probe in the
system is not hydrolyzed but reserved; (D) under ssDNA induction, silver ions are reduced to generate near
infrared fluorescent
silver nano clusters; the
system fluorescence intensity is detected, so that the content of the patulin in the to-be-tested sample is measured. The method has the advantages that the sensitivity is high, the operation is simple, the cost is low and the like.