Animal fresh semen low-temperature diluent as well as preparation method and application thereof

[0039] A preparation method for low-temperature diluent of fresh animal essence, comprising the steps of:

[0040] (1) Weigh 3g of analytically pure glucose and 3g of sodium citrate into 100mL of distilled water, stir until dissolved, then autoclave at 120°C for 30min, cool to obtain the base solution;

[0041] (2) For fresh eggs, use alcohol cotton balls with a volume fraction of 75% to sterilize the outer shell. After it is completely volatilized, break the egg, separate the egg white, egg yolk and frenulum, and put the egg yolk in half of the egg shell. inside, and carefully pour the egg yolk on a sterile paper towel folded in half (8 layers); carefully roll the egg yolk on the paper towel, so that the thin egg white on the surface is absorbed by the paper towel; Take a small mouth, and then use a 10mL disposable syringe with the needle removed, slowly suck the egg yolk from the small mouth, try to avoid inhaling the air bubbles, and at the same time avoid sucking the vitellin

[0046] A preparation method for low-temperature diluent of fresh animal essence, comprising the steps of:

[0047] (1) Weigh 5g of analytically pure glucose and 5g of sodium citrate into 100mL of distilled water, stir until dissolved, then autoclave at 120°C for 30min, cool to obtain the base solution;

[0048] (2) For fresh eggs, use alcohol cotton balls with a volume fraction of 75% to sterilize the outer shell. After it is completely volatilized, break the egg, separate the egg white, egg yolk and frenulum, and put the egg yolk in half of the egg shell. inside, and carefully pour the egg yolk on a sterile paper towel folded in half (8 layers); carefully roll the egg yolk on the paper towel, so that the thin egg white on the surface is absorbed by the paper towel; Take a small mouth, and then use a 10mL disposable syringe with the needle removed, slowly suck the egg yolk from the small mouth, try to avoid inhaling the air bubbles, and at the same time avoid sucking the vitellin

[0053] A preparation method for low-temperature diluent of fresh animal essence, comprising the steps of:

[0054] (1) Weigh 2g of analytically pure glucose and 2g of sodium citrate into 100mL of distilled water, stir until dissolved, then autoclave at 120°C for 30min, cool to obtain the base solution;

[0055] (2) For fresh eggs, use alcohol cotton balls with a volume fraction of 75% to sterilize the outer shell. After it is completely volatilized, break the egg, separate the egg white, egg yolk and frenulum, and put the egg yolk in half of the egg shell. inside, and carefully pour the egg yolk on a sterile paper towel folded in half (8 layers); carefully roll the egg yolk on the paper towel, so that the thin egg white on the surface is absorbed by the paper towel; Take a small mouth, and then use a 10mL disposable syringe with the needle removed, slowly suck the egg yolk from the small mouth, try to avoid inhaling the air bubbles, and at the same time avoid sucking the vitellin