13 results about "Saccharomyces" patented technology

A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant component with at least one probiotic. For example, the method may modify the asparagine content in tobacco materials, which can result in a modification in acrylamide production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant component treated according to the invention can be a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a portion thereof. Examples of probiotics include probiotic species of the genera bifidobacterium, lactobacillus, enterococcus, proionobacterium, bacillus, saccharomyces, streptococcus, and mixtures thereof. Smoking articles and other tobacco products including such probiotic-treated tobacco materials are also provided.

The invention provides a traditional Chinese medicine microbial ecological agent for preventing and treating infectious bursal disease of chicken and a preparation method thereof. The preparation method comprises the following steps: adding water to radix isatidis, honeysuckle, forsythia, folium isatidis, humulus scandens, radix scutellariae, dangshen, ageratum, plantain, astragalus membranaceus, angelica sinensis and liquorice, boiling by using high heat, preserving heat for 30-60 minutes by using slow fire, cooling, filtering, collecting filtrate, adding white sugar, boiling, cooling to a normal temperature, then inoculating lactobacillus, zygosaccharomyces bailii and acetobacter xylinum, and fermenting for 3-7 days so as to obtain the microbial ecological agent. After the microbial ecological agent is diluted proportionally, the diluted microbial ecological agent is directly drunk by chicken. The traditional Chinese medicine microbial ecological agent provided by the invention has the effects of resisting viruses, inducing interferon, increasing the quantity of effective microbial communities in intestinal tracts, boosting immunity and improving disease resistance of chickens without toxic or side effects and the like; the preparation method is simple, convenient to use and high in viable bacteria content and has no residues. The traditional Chinese medicine microbial ecological agent provided by the invention has a certain curative effect on infectious bronchitis and henpox and relatively good market prospect.

The invention relates to the field of feed, in particular to fiber-containing rumination fermented feed and a production method thereof. The method includes the steps: pretreating lignocellulose raw materials, mixing the pretreated materials with liquid by-products of the alcohol industry, mixing the obtained mixture with other raw materials so as to obtain a fermentation substrate, then inoculating a mixed bacterial liquid containing saccharomycetes, lactic acid bacteria and bacillus into the fermentation substrate, and performing fermentation so as to obtain the fiber-containing rumination fermented feed. Through the method, the problem of resource utilization of the lignocellulose raw materials can be solved, a new way to utilize the by-products of the alcohol industry can be provided,and the high-quality rumination fermented feed for animals can be produced. In addition, the fiber-containing rumination fermented feed which is produced by using the method has low content of neutraldetergent fiber and acid detergent fiber, good fermentation quality, a high nutritional value and better digestion characteristics, and can be widely used as high-quality rumination animal feed raw materials in animal husbandry.

The invention discloses a yeast strain capable of degrading L-malic acid in grape wine and application, and belongs to the technical field of wine brewing engineering. The L-malic acid degrading yeaststrain is screened from 316 yeast strains, the strain degrades malic acid in a liquid culture medium taking L-malic acid as a unique carbon source, and the deacidification rate is greater than 90%. Through identification, the yeast strain is Pichia kluyveri, and is named as Pichia kluyveri LI-27-1, and is preserved in China Center for Type Culture Collection (CCTCC) on July 29th, 2020, and the preservation number is CCTCC NO: M 2020372. The yeast strain can be mixed with saccharomyces cerevisiae for fermentation, so that malic acid in grape wine is reduced from 2.47 g/L to 1.70 g/L, the deacidification rate can reach 31.17%, and meanwhile, the flavor and quality of the grape wine can be improved.

The invention relates to a method for preparing a bacterial fertilizer from glutamic acid fermentation waste liquid and application of the bacterial fertilizer, belonging to the technical field of vegetable straw mulching. The method comprises the following steps: S1, collecting vegetable straw waste and then cutting the collected vegetable straw waste into sections so as to obtain straw waste A;S2, mixing the glutamic acid fermentation waste liquid, the straw waste A and water to obtain a mixture A; S3, adding saccharomycetes, lactic acid bacteria, bacillus subtilis and trichoderma aureoviride into the mixture A, and carryin gout natural fermenting to obtain a fermented material A; and S4, adding an organic nitrogen source into the fermented material A, and carrying out sealed fermentation to obtain the zymophyte fertilizer. The invention also provides the application of the zymophyte fertilizer prepared by using the method in promoting vegetable growth. According to the invention, the industrial glutamic acid fermentation waste liquid and agricultural vegetable straw waste can be treated at the same time, and the prepared zymophyte fertilizer is rich in nutrients and good in economic benefits.

The invention discloses a microbial preparation for treating vaginitis. The microbial preparation is prepared from the following components in parts by mass of 50-90 parts of water, 8-12 parts of molasses, 10-25 parts of saccharomycetes, 18-27 parts of bacillus, 45-55 parts of lactic acid bacteria, 5-10 parts of photosynthetic bacteria and 15-25 parts of bifidobacterium. The preparation method comprises the steps of 1, taking a certain amount of water, uniformly stirring the water and the molasses, heating to 121 DEG C, maintaining the pressure and preserving the heat for 30 minutes; 2, reducing the temperature to 35 DEG C; 3, adding a certain amount of saccharomycetes, bacillus, lactic acid bacteria, photosynthetic bacteria and bifidobacteria into the solution stirred in the step 2; and 4, enabling the microbial preparation prepared in the step 3 to be subpackaged and stored at the normal temperature. The preparation method is simple, low in cost, simple in storage method, good in vaginitis treatment effect, safe in clinical application, free of adverse reactions and convenient to popularize widely.

The invention discloses fructus lycii and chrysanthemum tea and a preparation method of same, wherein the tea includes the following raw materials, by mass, 1-5 parts of dry chrysanthemum morifolium, 10-18 parts of dry fructus lycii, 2-4 parts of astragalus membranaceus, 10-18 parts of honey, 8-14 parts of rock sugar and 1200-1800 parts of pure water. The preparation method includes the following steps: a) cutting the astragalus membranaceus into pieces being 0.2 mm in thickness and stir-frying the astragalus membranaceus on soft fire for 4-6 min for later use; b) milling the stir-fried astragalus membranaceus, the dry fructus lycii aned the dry chrysanthemum morifolium into powders for later use; c) mixing the milled powders in the step b) with 100-200 parts by mass of pure water and adding 3-5 parts of by mass of yeast to ferment the powders at 25-45 DEG C for 3-7 h; d) irradiating the fermented powders by ultraviolet for 5-10 min to perform sterilization; e) adding the honey, the rock sugar and the rest pure water and fully stirring the mixture until the rock sugar are melted completely; and f) fill-packaging the tea under a sterilized environment and sealing and storing the finish product. The active substances in the fructus lycii and chrysanthemum tea are easy to absorb by human body after fermentation and the tea is good in taste.

The invention discloses an efficient phenylamine degradable bacterial preparation and a preparation method thereof. The preparation comprises the following raw materials in percent by mass: 5-10% of pseudomonas putida, 10-15% of candida mycoderma bacteria, 7-13% of bacillus amyloliquefaciens, 6-11% of paper-soluble clostridum, 8-15% of trichoderma aureoviride, 2-6% of nitrobacterium, 4-7% of pseudomonas aeruginosa, 3-5% of bacillus subtilis, 11-16% of alcaligenes faecalis, 5-8% of rhodococcus and 4-9% of bacillus. A certain amounts of the prepared bacterial preparation is put into wastewater,then degradation of the bacterial preparation upon pollutants can be accelerated, and the bacterial preparation comprises multiple microorganisms with excellent refractory pollutant degradation capabilities, the strains are reasonably matched, coexist harmoniously, are not antagonized mutually, are high in activity, are large in biomass and rapid in propagation, and have a good degradation effecton phenylamine polluted water bodies when being used in wastewater.

The invention discloses a high ribonucleic acid saccharomyces cerevisiae strain and application thereof, and belongs to the technical field of bioengineering. According to the invention, the normal pressure and room temperature plasma mutagenesis technology (ARTP) is used for mutagenizing an original strain (9.20%), and the KS culture medium is used for primarily screening the mutagenic strain, so that the screening efficiency is greatly improved. The JNB-G03H8 with excellent characters is obtained by re-screening in the plurality of obtained high-yield ribonucleic acid yeasts. The RNA content of the strain is high, after shake-flask culture in a normal YPD culture medium, the RNA content (g/DCW) of the strain can reach 12.89%, the RNA content of the strain can reach 15.80% at most under the condition of an optimized culture medium through a response surface optimization experiment, compared with 9.20% of an original strain G03, the RNA content of the strain is improved by 70.76%, and the strain is a non-transgenic strain, non-toxic, harmless, stable in heredity and safe and reliable when applied to the field of food.

The invention belongs to the field of genetic engineering and discloses a genetic engineering strain for displaying Escherichia coli enterotoxin by Saccharomycetes and application of the genetic engineering strain. Fusion genes of Escherichia coli heat-resistant enterotoxins (ST) estA and estB after site-directed mutagenesis are connected into a surface display plasmid pYD1, the surface display plasmid pYD1 is converted into saccharomyces cerevisiae through electric shock, an ST-yeast genetic engineering strain is obtained through screening, and it is proved through immunofluorescence detection that an ST-fused protein is successfully displayed on the surface of the saccharomyces cerevisiae. Animal experiments prove that the engineering strain not only has a good probiotic function of the saccharomyces cerevisiae and can be used for improving the immunity of intestinal mucosa, but also can be used for effectively preventing animal intestinal flora imbalance and small intestinal mucosa structure damage caused by enterotoxigenic Escherichia coli, and has a specific immunoprophylaxis function on diseases such as animal diarrhea caused by the enterotoxigenic Escherichia coli.

The invention relates to the technical field of selenium yeast production, in particular to selenium yeast for feed and a preparation method of the selenium yeast. The invention relates to a preparation method of selenium yeast for feed, which comprises the following steps: step A, inoculating a strain of pichia pastoris preserved in a glycerol tube into an eggplant bottle seed culture medium, standing at 30-35 DEG C for eggplant bottle slant seed culture for 24-72 hours to obtain eggplant bottle slant seeds, the classification name of the pichia pastoris is pichia pastoris, and the classification name of the pichia pastoris is CGMCC (China General Microbiological Culture Collection Center); the pichia pastoris X33 PLT-07 strain is preserved in Guangdong Microbiological Culture Collection Center, and the preservation number is GDMCC No: 60262. The preparation method of the selenium yeast for the feed is simple, the prepared selenium yeast product is high in yield and low in production cost, the problems that an existing selenium yeast product is low in production yield and high in production cost are solved, and the prepared selenium yeast for the feed is high in organic selenium content and high in selenium content. The problem that selenium yeast prepared in the prior art is low in organic selenium content is solved.

The invention provides a biological fermentation feed taking weakly washed chicks as a main raw material, which is obtained by taking weakly washed chicks and molasses beancurd skin as fermentation base materials and carrying out solid fermentation on the fermentation base materials by using compound probiotics. The compound probiotics are two or more of saccharomycetes, lactobacillus plantarum and lactobacillus reuteri. The weight ratio of the weakly washed chicks to the molasses bean curd skin is 1: (1.5-3.5). According to the application of the biological fermentation feed taking the weakly washed chicks as the raw material, the addition amount of the biological fermentation feed in a breeding field is 4-20%. According to the invention, the high-quality animal protein source of the weakly washed chicks in the hatchery, the oligosaccharide prebiotics of the by-product molasses beancurd skin of the oil plant and the good adsorbability of the chicks and the oligosaccharide prebiotics are fully utilized and are subjected to mixed fermentation to prepare the high-quality fermented feed, so that the resources are efficiently utilized in a high-quality manner, the high-quality raw materials are prevented from being treated as organic fertilizers, the price is low, and the market prospect is wide.