High-yield production method of lovastatin

[0042] Mutation and preliminary screening of Monascus strain with high lovastatin production

[0043] Wash the spores from the growth slant of the initial strain Monascus purpureus with normal saline to obtain a spore suspension, inoculate the seed medium, and cultivate for about 36 hours. Take the bacterial suspension, filter it with sterile gauze, and shake it with glass beads Disperse to a single spore suspension and collect the single spore suspension for later use.

[0044] Add an equal volume of monospore suspension and 1% lithium chloride solution into a sterile test tube, mix well, let stand for 3 hours, and finally dilute 100 times with sterile phosphate buffer to terminate the reaction.

[0045] Draw 1 mL of the spore dilution treated with lithium chloride and inject it into a sterile small dish, and place it under laser irradiation. The irradiation time is 10 min. Laser wavelength: 632.8 nm, and the irradiation power density is 17.6 mw / cm2.

[0046] After the muta

[0048] Production of Lovastatin by Liquid Fermentation of Monascus

[0049] Fermentation strain: Monascus purpureus

[0050] Seed medium:

[0051] Rice flour 3 g, glucose 2 g, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0052] Fermentation medium:

[0053] Rice flour 7 g, glycerin 5 mL, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0054] Training conditions:

[0055] Monascus spores were scraped from the plate and transferred to a Erlenmeyer flask containing 50 mL of seed medium, and cultured at 30°C for 3 days. According to the inoculation amount of 15%, the grown seed culture medium was transferred to 150 mL fermentation medium, and cultured on a shaker at 150 r / m at 30°C for 3 days, then cultured on a shaker at 150 r / m at 24°C for 20 days.

[0056] Sample processing and testing:

[0057] After the fermentation broth was centrifuged at 10,000 g for 10 min,

[0060] Production of lovastatin by liquid state fermentation of monascus after mutagenesis of the present invention

[0061] Fermentation strain: Monascus purple

[0062] Seed Medium:

[0063] Rice flour 3 g, glucose 2 g, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0064] Fermentation medium:

[0065] Rice flour 7 g, glycerin 5 mL, NaNO 3 0.2 g, peptone 1.5 g, MgSO 4 ·7H 2 O 0.05 g, KH 2 PO 4 0.15 g, add water to 100 mL.

[0066] Training conditions:

[0067] The spores of Monascus MPB3 were scraped from the plate and transferred to a Erlenmeyer flask containing 50 mL of seed medium, and cultured at 30°C for 3 days. According to the inoculation amount of 15%, the grown seed culture medium was transferred to 150 mL fermentation medium, and cultured on a shaker at 150 r / m at 30°C for 3 days, and then cultured on a shaker at 150 r / m at 24°C for 20 days.

[0068] Sample processing and testing:

[0069] After t