Technology for spraying breeding of dendrobium huoshanense cells

一种霍山石斛、细胞的技术,应用在将霍山石斛细胞进行喷涂育种领域,能够解决石斛繁殖能力低、无法满足大面积野化的需要等问题

Inactive Publication Date: 2021-08-10
陈大卫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to alleviate the endangered situation of wild Dendrobium, artificially cultivated Dendrobium seedlings are currently used for wild treatment, but the reproductive capacity of Dendrobium itself is low and cannot meet the needs of large-scale wildization

Method used

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0026] In describing the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", " The orientation or positional relationship indicated by "outside", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, so as to Specific orientation configurations and operations, therefore, are not to be construed as limitations on the invention.

[0027] The embodiment tha...

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Abstract

The invention discloses a technology for spraying breeding of dendrobium huoshanense cells. The technology comprises the following steps: firstly, selecting dendrobium huoshanense branches with high plants and thick stems, washing and disinfecting the dendrobium huoshanense branches, dissolving small dendrobium huoshanense branches by using cellulase, after oscillation by a shaking table and centrifugation by a centrifugal machine, conducting filtering to obtain the dendrobium huoshanense cells, and finally, spraying the dendrobium huoshanense cells into a culture medium for breeding. According to the technology for the spraying breeding of the dendrobium huoshanense cells, cellulase and pectinase are added during cell separation, the dendrobium huoshanense cells can be rapidly separated from the dendrobium huoshanense branches, coconut juice and potato juice are added into the culture medium, the coconut juice can promote cell division, the potato juice can buffer the PH of the culture medium, strong plants are obtained, the dendrobium huoshanense is bred through the cells, so that a large quantity of the pure dendrobium huoshanense young plants can be rapidly obtained, and the demand of the market on the dendrobium huoshanense can be alleviated after the wild treatment.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a technique for spraying and breeding cells of Dendrobium huoshanense. Background technique [0002] Among the 76 kinds of Dendrobium plants in China, there are more than 40 kinds of medicinal ones. Its pharmacology is mainly to strengthen yin and benefit body fluid, tonify kidney and benefit strength, improve eyesight and prolong life. However, due to a large number of harvesting, and the growth of Dendrobium is very slow and the growth cycle is long, wild Dendrobium is quite rare, and even if there are, it is difficult to have good quality. [0003] Dendrobium Huoshanense, also known as Mihu, is a plant of the genus Dendrobium in the family Orchidaceae, and is the top grade of medicinal Dendrobium. Dendrobium Huoshanense is extremely demanding on growing conditions. Due to long-term human destruction and indiscriminate harvesting, the wild plants of Dendrobium are on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 陈大卫
Owner 陈大卫
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