Application of beta-glucan and succinyl glycan in prevention and control of soil-borne fungal diseases

[0033] Determination results of relative abundance of Streptomyces in typical soil

[0034] The primeval forest land in Enshi Prefecture, Hubei Province, the rotational cropland in Nanjing City, Jiangsu Province, the continuous potato cropping land in Tengzhou City, Shandong Province, and the continuous tobacco cropping land in Kunming City, Yunnan Province were selected as test areas. The mixture powder of β-glucan and succinoglycan (laminaran:succinoglycan=1:1w / w) was added to the selected soil surface, and the usage amounts were 0%, 0.05%, and 0.25%, respectively. Then, take a sample every 15d and end at 60d. The sampled soil was extracted with the Ezup Column Soil DNA Extraction Kit (Sangon Biotech (Shanghai)) for total soil DNA and soil metagenomic sequencing. According to the sequencing results, the relative abundance of Streptomyces sequences in the soil microbial flora was analyzed. Figure 1-Figure 4 The trends of the relative abundance of Streptomyces in the above f

[0036] Changes of activities of β-glucanase, cellulase and chitinase in soil

[0037] Select the rotation cultivated land in Nanjing City, Jiangsu Province as the test soil sample, add β-glucan and succinoglycan (yeast glucan: succinoglycan = 1:10w / w) mixture powder to the selected soil surface, use The amounts are 0%, 0.05%, 0.25%, respectively. Then, take a sample every 15d and end at 60d. Using laminarin, sodium carboxymethyl cellulose and chitin colloid as substrates, the hydrolase activity of the soil extract was measured at pH 5.5, the reaction temperature was 35°C, and the reaction time was 24 hours. Figure 5-Figure 7 It is the change trend of the three hydrolytic enzyme activities in the soil with time. It can be seen from the figure that after the mixture of β-glucan and succinoglycan was added, the activities of the three enzymes all increased significantly with time and the amount of the mixture added. The results showed that adding the mixture of β-glucan and su

[0039] Soil fungus content determination results

[0040] A potato continuous cropping field in Tengzhou City, Shandong Province was selected as a soil sample, and a mixture of β-glucan and succinoglycan (lentinan: succinoglycan=1:0.2w / w) was prepared into a solution with a concentration of 25g / L. Then spray to the selected soil surface, and the dosage is 0%, 0.05%, 0.25%. Then, take a sample every 15d and end at 60d. Then, the abundance change process of cultivable fungi in the soil was determined by the dilution plate counting method: take 1 g of soil sample and dilute it into 10 mL of sterile phosphate buffer, shake vigorously for 30 minutes to make the soil evenly dispersed in the buffer, and then carry out gradient dilution , and finally select the appropriate concentration and spread it on the PDA plate, and count after culturing at 30°C for 48 hours. Figure 8 As shown, the content of fungi in the soil gradually decreased with the increase of the addition time and po