Proliferation culture medium formula in blueberry tissue culture

A proliferation medium and tissue culture technology, applied in the field of plant tissue culture, can solve the problems of long rooting time, low reproduction coefficient, high management cost, etc., and achieve the effect of easy preparation, short time and high proliferation efficiency

Inactive Publication Date: 2009-09-23
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology has several benefits compared with traditional methods such as mixing different materials together or adding other chemicals like fertilizers during cultivating processes. It also allows for easier preparations without requiring specific forms due to its use of commonly available sources of raw material and high growth rates per unit area. Additionally it provides fast-growth cultures at very low cost while maintaining their ability to produce roots quickly enough for optimal plantings.

Problems solved by technology

The technical problem addressed by this patented technology relates to providing methods or compositions for promoting growth of plants during plant cultivations without causing damage caused by weather conditions at different locations where crops may also exist throughout their entire life cycle (temperature-humidity). This would help improve productivity while minimizing costs associated with replantings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In March, take lingonberry sharpblue (the current production variety) about 15 cm from the middle and upper part of the current year's branches as explants, rinse with tap water for 15 minutes, gently scrub with gauze to remove stains, rinse with tap water for 15 minutes, and cut the rinsed branches into Small sections of 4-5cm, with 2-4 buds left for each section, put them in an ultra-clean workbench for disinfection. Disinfection procedure: 75% ethanol for 30 seconds, 0.1% mercury liter for 15 minutes (add 2-3 drops of Tween 80) and shake from time to time, then rinse with sterile water 4 times, 3 minutes each time. Cut off 0.5cm at both ends of the sterilized branches, then cut the branches into small sections of 1-2cm, keep 1-2 buds in each section, and finally inoculate the cut stem sections with buds into the pre-prepared added 0.5mg.L -1 On ordinary MS medium of ZT, culture at 25°C under 1500-2500Lex light for 2 weeks, and the light time is 12 hours / day. After 2

Embodiment 2

[0028] In March, take the middle and upper part of the lingonberry No. 3 variety (the current production variety) as explants, rinse with tap water for 15 minutes, gently scrub with gauze to remove stains, and then rinse with tap water for 15 minutes. Cut the final branches into small sections of 4-5cm, leave 2-4 buds in each section, and put them in an ultra-clean workbench for disinfection. Disinfection procedure: 75% ethanol for 30 seconds, 0.1% mercury liter for 15 minutes (add 2-3 drops of Tween 80) and shake from time to time, then rinse with sterile water 4 times, 3 minutes each time. Cut off 0.5cm at both ends of the sterilized branches, then cut the branches into small sections of 1-2cm, keep 1-2 buds in each section, and finally inoculate the cut stem sections with buds into the pre-prepared added 0.5mg.L -1 On ordinary MS medium of ZT, culture at 25°C under 1500-2500Lex light for 2 weeks, and the light time is 12 hours / day. After 2 weeks, the germinated shoots were

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PUM

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Abstract

The invention relates to a proliferation culture medium formula in blueberry tissue culture, which consists of the components with different concentrations as follows: macroelements, microelements, iron salts, organic compositions and the like. By using the culture medium, the proliferation efficiency can be as high as more than 15 times; and the proliferation culture time is as short as only 25-30 days, and proliferation seedlings can reach 3-4cm in height, thus fully satisfying the need for rooting culture. Simultaneously, the culture medium consists of the compositions which have common sources without compounds with special forms, and has easy preparation.

Description

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Claims

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Application Information

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Owner SOUTHWEST UNIVERSITY
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