Culture medium and culture method for culturing haematococcus pluvialis by using brewery wastewater

A technology of Haematococcus pluvialis and a culturing method is applied in the culture medium and the field of culturing Haematococcus pluvialis, and can solve the problems of high efficiency, low astaxanthin content, slow growth and the like

Inactive Publication Date: 2014-08-06
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In short, UASB has the advantages of high efficiency, low cost, low power consumption, less investment, and less land occupation, but the disadvantage is that the concentration of CODcr in the effluent is still high, reaching 500mg / L
[0008] The cultivation of Haematococcus pluvialis traditionally uses BBM medium and open cement ponds, which has slow growth, low yield, low astaxanthin content, large investment, easy pollution, and low benefit

Method used

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Embodiment Construction

[0021] 1) Beer wastewater pretreatment method: Beer wastewater needs to be pretreated before it is used to grow algae. The method is: first filter the wastewater with a 60-mesh sieve to remove floating objects, glass slag, hair, sediment, leaves, plastic bags, etc. For larger solids, the sewage is introduced into the cement aeration tank with a length of 30 meters, a width of 15 meters and a depth of 0.8 meters. The pool is oriented east-west to receive more sunlight; the water depth is kept at 65 cm; Add 45 grams of quicklime to the sewage and sprinkle quicklime evenly (it is best to add 1 kg of quicklime to 5 liters of pure water in advance, dissolve, dilute, stir in a plastic bucket, and then sprinkle the whole pool), every 30 minutes, and then press the amount of water per cubic meter. Add 25 grams of rice sewage to the whole pool and sprinkle alum (alum is preferably dissolved in a plastic bucket in the amount of 100 grams plus 2 liters of pure water in advance, diluted an...

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Abstract

The invention relates to a culture medium for culturing haematococcus pluvialis by using brewery wastewater, of which the formula is as follows: 80 ml of brewery wastewater, 28 mg of sodium citrate, 20 mg of urea, 12 mg of potassium dihydrogen phosphate, 150 mg of fructose, 45 mg of yeast extract powder, 50 mg of casein tryptone, 12 mg of ammonium citrate, 25 mg of whey powder, 30 mg of lactose, 60 mg of glucose, 1.8 ml of horse manure leachate, 2 ml of fermented soya-bean milk, 100 mg of sorbitol, 24 mg of soluble starch, 25 mg of soytone, 28 mg of beta-sodium glycerophosphate, 12 mg of an edetic acid, 10 mcg of vitamin B1, 21 mcg of vitamin B1, 1.8 ml of soil extract, 1.5 ml of human urine, 2.4 ml of chicken manure leachate, and 1000 ml of pure fresh water disinfected by sodium hypochlorite. An important bioactive substance-astaxanthin can be obtained. The culture medium is low in cost, uneasily polluted, and can be reused; and due to the mixed utilization of organic fertilizers and inorganic fertilizers, the growth rate and astaxanthin content of haematococcus pluvialis are significantly increased, and the yield is increased by 270%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a culture medium and a culture method for cultivating Haematococcus pluvialis by using wastewater from a brewery. Background technique [0002] The wastewater produced in the beer brewing process has the characteristics of large amount of wastewater, high content of suspended solids and organic matter. Wastewater itself is not toxic, but a large amount of biodegradable organic matter contained in it is directly discharged into the water body, which will consume a large amount of dissolved oxygen in the water, resulting in hypoxia in the water body, resulting in the death of aquatic organisms including fish, and the waste water contains a large amount of N and P inorganic substances. Salt causes eutrophication of water bodies, deteriorates water quality, and pollutes the environment. Beer wastewater is divided into three parts: one is cooling water, accounting for 70% of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner LINYI UNIVERSITY
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