Culturing method for mycelia of Antrodia camphorata

[0028] Prepare each raw material to prepare slant medium according to the following formula:

[0029] Potato 100g / L, glucose 10g / L, corn flour 10g / L, peptone 2g / L, magnesium sulfate 0.1g / L, potassium dihydrogen phosphate 0.2g / L, VB 1 10mg / L, agar powder 16g / L;

[0030] Inoculate the strain of Antrodia camphorata on the slant medium prepared above for activation and purification, and the culture temperature is 25°C to obtain the activated mother species;

[0031] Prepare each raw material according to the following formula to prepare liquid shake flask culture medium:

[0032] Potato juice 10g / 100mL, glucose 1g / 100mL, corn flour 1g / 100mL, peptone 0.2g / 100mL, magnesium sulfate 0.01g / 100mL, potassium dihydrogen phosphate 0.02g / 100mL, VB 1 0.001g / 100mL and agar powder 0.05g / 100mL;

[0033] Inoculate the above-mentioned activated mother species into the liquid shake flask culture medium for shake flask culture. The culture temperature of the above s

[0043] Prepare each raw material to prepare slant medium according to the following formula:

[0044] Potato 200g / L, glucose 15g / L, corn flour 30g / L, peptone 4g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 1g / L, VB 1 30mg / L, agar powder 18g / L;

[0045] Inoculate the strains of Antrodia camphorata on the slant medium prepared above for activation and purification, and the culture temperature is 28°C to obtain the activated mother species;

[0046] Prepare each raw material according to the following formula to prepare liquid shake flask culture medium:

[0047] Potato juice 20g / 100mL, glucose 1g / 100mL, corn flour 3g / 100mL, peptone 1g / 100mL, magnesium sulfate 0.05g / 100mL, potassium dihydrogen phosphate 0.1g / 100mL, VB 1 0.005g / 100mL and agar powder 0.1g / 100mL;

[0048] Inoculate the above-mentioned activated mother species into the liquid shake flask culture medium for shake flask culture. The culture temperature of the above shake

[0059] Prepare each raw material to prepare slant medium according to the following formula:

[0060] Potato 200g / L, glucose 20g / L, corn flour 50g / L, peptone 5g / L, magnesium sulfate 1g / L, potassium dihydrogen phosphate 2g / L, VB 1 50mg / L, agar 20g / L;

[0061] Inoculate the strains of Antrodia camphorata on the slant medium prepared above for activation and purification, and the culture temperature is 30°C to obtain the activated mother species;

[0062] Prepare each raw material according to the following formula to prepare liquid shake flask culture medium:

[0063] Potato juice 50g / 100mL, glucose 4g / 100mL, corn flour 5g / 100mL, peptone 1g / 100mL, magnesium sulfate 0.1g / 100mL, potassium dihydrogen phosphate 0.2g / 100mL, VB 1 0.01g / 100mL and agar powder 0.15g / 100mL;

[0064] Inoculate the above-mentioned activated mother species into the liquid shake flask culture medium for shake flask culture. The culture temperature of the above shake flask cult