4 results about "Shake flask" patented technology

The invention discloses a culturing method for the mycelia of Antrodia camphorata, belonging to the field of biological fermentation technology. The method of the invention comprises the following steps: inoculating a slant culture medium with a Antrodia camphorata strain for activation and purification so as to obtain an activated mother strain; inoculating a liquid shake-flask culture medium with the activated mother strain for static culture, and carrying out stirring culture after mycelia germinate and are free of pollution so as to obtain a shake-flask strain; inoculating a fermentation medium with the shake-flask strain for fermentation culture so as to obtain fermented mycelia; and inoculating a artificially synthesized solid culture medium with the fermented mycelia which are usedas a liquid strain and carrying out fermentation for 30 to 90 days so as to obtain the mycelia of Antrodia camphorata. The method of the invention has the advantages of a short cultivation period forthe mycelia of Antrodia camphorata, a high culture success rate of and high medicinal value of the mycelia.

The invention discloses a mutagenesis method for collagenogenesis yeast on the basis of high-magnetic field gravity environment, wherein the collagenogenesis yeast is mutated by using the high-magnetic field gravity environment generated by a ground simulation apparatus. The method includes: placing a collagenogenesis yeast strain, in logarithmic phase, under the high-magnetic field gravity environment, wherein magnetic flux density is 12-16 T, gravity level is 0-2 g, and mutagenesis time is 24-72 h, thus obtaining mutated strain; coating a solid culture medium with the mutated strain, selecting single colony with vigorous growth, inoculating the colony on a liquid culture medium and performing shaking flask fermentation, thereby obtaining a high-collagen yield strain with yield of collagen being detection standard. According to the invention, by means of the high-magnetic field gravity environment generated by the ground simulation apparatus for mutagenesis, the method is simple in operation and high in efficiency, is good in screening effect and is free of environment pollution. Fatigue effect of the bacterial strain on regular physical and chemical mutagenesis methods is also relieved.